验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HEK-293 cells, human testis tissue, mouse ovary tissue, mouse thymus tissue, PC-3 cells, rat brain tissue, mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20878-1-AP targets LZTS1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag14977 Product name: Recombinant human LZTS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 397-596 aa of BC075007 Sequence: QLKESQTEVNAKASEILGLKAQLKDTRGKLEGLELRTQDLEGALRTKGLELEVCENELQRKKNEAELLREKVNLLEQELQELRAQAALARDMGPPTFPEDVPALQRELERLRAELREERQGHDQMSSGFQHERLVWKEEKEKVIQYQKQLQQSYVAMYQRNQRLEKALQQLARGDSAGEPLEVDLEGADIPYEDIIATEI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | leucine zipper, putative tumor suppressor 1 |
| 别名 | F37, F37/esophageal cancer-related gene-coding leucine-zipper motif, Fez1 |
| 计算分子量 | 596 aa, 67 kDa |
| 观测分子量 | 67 kDa |
| GenBank蛋白编号 | BC075007 |
| 基因名称 | LZTS1 |
| Gene ID (NCBI) | 11178 |
| RRID | AB_10693683 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y250 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LZTS1 (Leucine Zipper Tumor Suppressor 1), also known as FEZ1, is a tumor suppressor protein that plays a critical role in regulating cell cycle progression and maintaining microtubule stability. It is frequently downregulated in various human cancers, including breast, gastric, and prostate cancer. LZTS1 interacts with the anaphase-promoting complex/cyclosome (APC/C) to promote the degradation of key mitotic regulators such as cyclin B1, thereby controlling the G2/M transition. Additionally, it influences cytoskeletal dynamics by binding to microtubules and actin filaments, affecting cell adhesion, migration, and proliferation. Loss or reduced expression of LZTS1 is associated with aggressive tumor behavior, increased metastatic potential, and poor clinical prognosis, highlighting its importance as a potential biomarker and therapeutic target in oncology.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for LZTS1 antibody 20878-1-AP | Download protocol |
| IHC protocol for LZTS1 antibody 20878-1-AP | Download protocol |
| IP protocol for LZTS1 antibody 20878-1-AP | Download protocol |
| WB protocol for LZTS1 antibody 20878-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Lzts1 controls both neuronal delamination and outer radial glial-like cell generation during mammalian cerebral development.
| ||
Mol Oncol Ryanodine receptor 2 promotes colorectal cancer metastasis by the ROS/BACH1 axis | ||
Int Dent J Screening Differentially Expressed Proteins in Areca Nut-Related Oral Squamous Cell Carcinoma Using Tandem Mass Tag Proteomics |
