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  • Featured Product
  • KD/KO Validated

LZTFL1 Polyclonal antibody

LZTFL1 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 17073-1-AP

Print datasheet

Synonyms

LZTFL1

验证数据展示

  • WB analysis using 17073-1-AP

    WB analysis using 17073-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17073-1-AP (LZTFL1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 17073-1-AP

    IHC staining of human testis using 17073-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17073-1-AP (LZTFL1 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human heart using 17073-1-AP

    IHC staining of human heart using 17073-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17073-1-AP (LZTFL1 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 17073-1-AP

    IF Staining of HepG2 using 17073-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 17073-1-AP (LZTFL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human lung using 17073-1-AP

    IHC staining of human lung using 17073-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 17073-1-AP (LZTFL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 17073-1-AP

    IHC staining of human colon using 17073-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17073-1-AP (LZTFL1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 17073-1-AP

    IHC staining of mouse heart using 17073-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17073-1-AP (LZTFL1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse lung using 17073-1-AP

    IHC staining of mouse lung using 17073-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 17073-1-AP (LZTFL1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 17073-1-AP

    IHC staining of mouse testis using 17073-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17073-1-AP (LZTFL1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat heart using 17073-1-AP

    IHC staining of rat heart using 17073-1-AP

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 17073-1-AP (LZTFL1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat small intestine using 17073-1-AP

    IHC staining of rat small intestine using 17073-1-AP

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 17073-1-AP (LZTFL1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat testis using 17073-1-AP

    IHC staining of rat testis using 17073-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 17073-1-AP (LZTFL1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human small intestine using 17073-1-AP

    IHC staining of human small intestine using 17073-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 17073-1-AP (LZTFL1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, U-251 cells, mouse testis tissue, rat testis tissue
Positive IHC detected inhuman heart tissue, human lung tissue, human colon tissue, human testis tissue, mouse heart tissue, mouse lung tissue, mouse small intestine tissue, mouse testis tissue, rat heart tissue, rat small intestine tissue, rat testis tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17073-1-AP targets LZTFL1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB, IP, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen LZTFL1 fusion protein Ag10919 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name leucine zipper transcription factor-like 1
Synonyms LZTFL1
Calculated Molecular Weight 299 aa, 35 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC025988
Gene Symbol LZTFL1
Gene ID (NCBI) 54585
RRIDAB_10950400
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NQ48
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LZTFL1 belongs to the LZTFL1 family. It is an important regulator of BBSome ciliary trafficking and hedgehog signaling. LZTFL1 regulates Ciliary Trafficking of the BBSome and Smoothened.(PMID:22072986)

实验方案

Product Specific Protocols
WB protocol for LZTFL1 antibody 17073-1-APDownload protocol
IHC protocol for LZTFL1 antibody 17073-1-APDownload protocol
IF protocol for LZTFL1 antibody 17073-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Dev Cell

IFT27 Links the BBSome to IFT for Maintenance of the Ciliary Signaling Compartment.

Authors - Thibaut Eguether
  • KO Validated
View Article
mouseWB,IF

EMBO J

Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.

Authors - Shichao Duan
View Article
mouseIF

J Cell Biol

Ubiquitin links smoothened to intraflagellar transport to regulate Hedgehog signaling.

Authors - Paurav B Desai
View Article
humanWB,IHC,IP

Oncogene

LZTFL1 inhibits kidney tumor cell growth by destabilizing AKT through ZNRF1-mediated ubiquitin proteosome pathway

Authors - Jun Lu
View Article