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验证数据展示

  • WB analysis using 32649-1-AP

    WB analysis using 32649-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32649-1-AP (LYVE-1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis using 32649-1-AP

    WB analysis using 32649-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32649-1-AP (LYVE-1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

LYVE1 Polyclonal antibody

LYVE1 Polyclonal Antibody for WB, ELISA
Cat No. 32649-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

Cell surface retention sequence-binding protein 1, CRSBP 1, CRSBP1, CRSBP-1, Extracellular link domain-containing protein 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse spleen tissue, bEnd.3 cells, rat spleen tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32649-1-AP targets LYVE1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Eg1615

Product name: Recombinant Human LYVE-1 protein (rFc Tag)(HPLC verified)

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 20-238 aa of NM_006691.4

Sequence: LVQGSLRAEELSIQVSCRIMGITLVSKKANQQLNFTEAKEACRLLGLSLAGKDQVETALKASFETCSYGWVGDGFVVISRISPNPKCGKNGVGVLIWKVPVSRQFAAYCYNSSDTWTNSCIPEIITTKDPIFNTQTATQTTEFIVSDSTYSVASPYSTIPAPTTTPPAPASTSIPRRKKLICVTEVFMETSTMSTETEPFVENKAAFKNEAAGFGGVPT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lymphatic vessel endothelial hyaluronan receptor 1
别名 Cell surface retention sequence-binding protein 1, CRSBP 1, CRSBP1, CRSBP-1, Extracellular link domain-containing protein 1
计算分子量35kDa
观测分子量55 kDa
GenBank蛋白编号NM_006691.4
基因名称 LYVE1
Gene ID (NCBI) 10894
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y5Y7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LYVE1 is a 322-amino acid type-I integral membrane glycoprotein primarily expressed on lymphatic endothelial cells. LYVE1 is a CD44 homologue. It acts as a receptor and binds to both soluble and immobilized hyaluronan. LYVE1 may function in lymphatic hyaluronan transport and have a role in tumor metastasis.

实验方案

Product Specific Protocols
WB protocol for LYVE1 antibody 32649-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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