验证数据展示
经过测试的应用
| Positive IP detected in | mouse heart tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31548-1-AP targets LYRM5 in IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IP, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag35889 Product name: Recombinant human LYRM5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-88 aa of BC071769 Sequence: MANSLRGEVLKLYKNLLYLGRDYPKGADYFKKRLKNIFLKNKDVKNPEKIKELIAQGEFVMKELEALYFLRKYRAMKQRYYSDTNKTN 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | LYR motif containing 5 |
| 别名 | ETFRF1 |
| 观测分子量 | 11 kDa |
| GenBank蛋白编号 | BC071769 |
| 基因名称 | LYRM5 |
| Gene ID (NCBI) | 144363 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q6IPR1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LYRM5, also known as ETFRF1, belongs to the Complex1_LYR-like Superfamily. LYRM5 is a mitochondrial protein and plays key roles in acetate metabolism, and in essential Fe-S cluster biogenesis. In vitro, LYRM5 was found to inhibit ETF by promoting the removal of flavin from the ETF holoenzyme, thus potentially regulating the rate of β-oxidation (PMID: 35402183, PMID: 36266680). The calculated molecular weight of LYRM5 is 11 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for LYRM5 antibody 31548-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
