验证数据展示
经过测试的应用
| Positive IP detected in | K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15946-1-AP targets LYL1 in IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IP, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | LYL1 fusion protein Ag8528 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | lymphoblastic leukemia derived sequence 1 |
| 别名 | Protein lyl-1, Lymphoblastic leukemia-derived sequence 1, Class A basic helix-loop-helix protein 18, bHLHa18 |
| 计算分子量 | 30 kDa |
| 观测分子量 | 35-40 kDa |
| GenBank蛋白编号 | BC002796 |
| 基因名称 | LYL1 |
| Gene ID (NCBI) | 4066 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P12980 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Lymphoblastic leukemia1 (LYL1) is a proto-oncogenic transcription factor found upregulated in patients with T-cell acute lymphoblastic leukemia (T-cell ALL). LYL1 has an important role in hematopoietic stem cell biology, normal hematopoiesis and leukemia. It is expressed throughout the hematopoietic lineages with the exception of T-cells. LYL1 shows two bands in the WB detection, where the upper band is the phosphorylated form and the lower band is the non-phosphorylated form (PMID: 10023675, PMID: 20844761).
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for LYL1 antibody 15946-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |