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验证数据展示

  • WB analysis of human testis using 12026-1-AP

    WB analysis of human testis using 12026-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 12026-1-AP (LY6K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of PC-3 using 12026-1-AP

    IP experiment of PC-3 using 12026-1-AP

    IP result of anti-LY6K (IP:12026-1-AP, 4ug; Detection:12026-1-AP 1:300) with PC-3 cells lysate 3200ug.

  • IHC staining of human skin cancer using 12026-1-AP

    IHC staining of human skin cancer using 12026-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 12026-1-AP (LY6K antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

LY6K Polyclonal antibody

LY6K Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 12026-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, IP, ELISA

别名

Lymphocyte antigen 6K, Ly-6K, Ly 6K, HSJ001348, CO16

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman testis tissue
Positive IP detected inPC-3 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12026-1-AP targets LY6K in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性bovine
免疫原

CatNo: Ag2656

Product name: Recombinant human LY6K protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-179 aa of BC001291

Sequence: GAPRADPPWAPLGTMALLALLLVVALPRVWTDANLTARQRDPEDSQRTDEGDNRVWCHVCERENTFECQNPRRCKWTEPYCVIAAVKIFPRFFMVAKQCSAGCAAMERPKPEEKRFLLEEPMPFFYLKCCKIRYCNLEGPPINSSVFKEYAGSMGESCGGLWLAILLLLASIAAGLSLS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lymphocyte antigen 6 complex, locus K
别名 Lymphocyte antigen 6K, Ly-6K, Ly 6K, HSJ001348, CO16
计算分子量 179 aa, 20 kDa
观测分子量 28 kDa
GenBank蛋白编号BC001291
基因名称 LY6K
Gene ID (NCBI) 54742
RRIDAB_2877817
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ17RY6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LY6K antibody 12026-1-APDownload protocol
IHC protocol for LY6K antibody 12026-1-APDownload protocol
IP protocol for LY6K antibody 12026-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
bovineWB

Int J Biol Macromol

TMT-based quantitative N-glycoproteomic analysis reveals glycoprotein protection can improve the quality of frozen bovine sperm.

Authors - Na Wang
View Article