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验证数据展示

  • IHC staining of human testis using 23933-1-AP

    IHC staining of human testis using 23933-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 23933-1-AP (LY6H Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 23933-1-AP

    IHC staining of human testis using 23933-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 23933-1-AP (LY6H Antibody) at dilution of 1:50 (under 40x lens).

  • WB analysis of Raji using 23933-1-AP

    WB analysis of Raji using 23933-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 23933-1-AP (LY6H Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

LY6H Polyclonal antibody

LY6H Polyclonal Antibody for WB, IHC, ELISA
Cat No. 23933-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Ly 6H, LY6H, Lymphocyte antigen 6H

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRaji cells
Positive IHC detected inhuman testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23933-1-AP targets LY6H in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 LY6H fusion protein Ag20762 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lymphocyte antigen 6 complex, locus H
别名 Ly 6H, LY6H, Lymphocyte antigen 6H
计算分子量 15 kDa
观测分子量 16 kDa
GenBank蛋白编号BC028894
基因名称 LY6H
Gene ID (NCBI) 4062
RRIDAB_2879366
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDO94772
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LY6H is highly expressed in particular subdivisions of human brain and also in MOLT-3 and -4 acute lymphoblastic leukemia cells. It has some isoforms with MW 14-16 kDa.

实验方案

Product Specific Protocols
WB protocol for LY6H antibody 23933-1-APDownload protocol
IHC protocol for LY6H antibody 23933-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols