验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human ovary tumor tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17483-1-AP targets LUZP1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag11518 Product name: Recombinant human LUZP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-358 aa of BC051733 Sequence: MAEFTSYKETASSRHLRFKLQSLSRRLDELEEATKNLQKAEDELLDLQDKVIQAEGSNSSMLAEIEVLRQRVLRIEGKDEEIKRAEDLCRLMKEKLEEEENLTRELKSEIERLQKRMAELEKLEEAFSRSKNDCTQLCLSLNEERNLTKKISSELEMLRVKVKELESSEDRLDKTEQSLASELEKLKSLTLSFVSERKYLNEKEKENEKLIKELTQKLEQNKKMNRDYTRNASNLERNDLRIEDGISSTLPSKESRRKGGLDYLKQVENETRNKSENEKNRNQEDNKVKDLNQEIEKLKTQIKHFESLEEELKKMKSKNNDLQDNYLSEQNKNKLLASQLEEIKLQIKKQKELENGEV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | leucine zipper protein 1 |
| 别名 | LUZP |
| 计算分子量 | 1026 aa, 115 kDa |
| 观测分子量 | 120 kDa |
| GenBank蛋白编号 | BC051733 |
| 基因名称 | LUZP1 |
| Gene ID (NCBI) | 7798 |
| RRID | AB_2139498 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q86V48 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LUZP1, also known as LUZP, is predominantly expressed in the brain. LUZP1 is a centrosome, actin, and midbody-localizing protein implicated in ciliogenesis regulation and actin cytoskeleton stability (PMID: 33869174). LUZP1 null mice present defective neural tube closure and cardiovascular problems, which cause perinatal death. In addition, LUZP1 has also been implicated in the etiology of diseases like the 1p36 and the Townes-Brocks syndromes (PMID: 35738212).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for LUZP1 antibody 17483-1-AP | Download protocol |
| IP protocol for LUZP1 antibody 17483-1-AP | Download protocol |
| WB protocol for LUZP1 antibody 17483-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Adv Res Leucine zipper protein 1 attenuates pressure overload-induced cardiac hypertrophy through inhibiting Stat3 signaling
| ||
Redox Biol Leucine zipper protein 1 prevents doxorubicin-induced cardiotoxicity in mice
| ||
Mol Cell Histone chaperone HIRA, promyelocytic leukemia protein, and p62/SQSTM1 coordinate to regulate inflammation during cell senescence. | ||
Sci Adv CEP76 impairment at the centrosome-cilium interface contributes to a spectrum of ciliopathies. | ||
Oncol Rep NF‑κB‑driven LUZP1 promotes metastasis and chemoresistance in head and neck squamous cell carcinoma.
| ||
J Neurosci LUZP1 Regulates Dendritic Spine Maturation and Synaptic Plasticity in the Hippocampal Dentate Gyrus of Mice.
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