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验证数据展示

  • WB analysis using 83585-1-RR (same clone as 83585-1-PBS)

    WB analysis using 83585-1-RR (same clone as 83585-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83585-1-RR (LUC7L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83585-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83585-1-RR (same clone as 83585-1-PBS)

    IF Staining of HepG2 using 83585-1-RR (same clone as 83585-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LUC7L antibody (83585-1-RR, Clone: 240311B10 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83585-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 83585-1-RR (same clone as 83585-1-PBS)

    FC experiment of MCF-7 using 83585-1-RR (same clone as 83585-1-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug LUC7L Recombinant antibody (83585-1-RR, Clone:240311B10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83585-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 83585-1-RR (same clone as 83585-1-PBS)

    IHC staining of rat brain using 83585-1-RR (same clone as 83585-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83585-1-RR (LUC7L antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83585-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 83585-1-RR (same clone as 83585-1-PBS)

    IHC staining of rat brain using 83585-1-RR (same clone as 83585-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83585-1-RR (LUC7L antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83585-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 83585-1-RR (same clone as 83585-1-PBS)

    WB analysis of HepG2 using 83585-1-RR (same clone as 83585-1-PBS)

    WB result of LUC7L antibody (83585-1-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LUC7L transfected HepG2 cells. This data was developed using the same antibody clone with 83585-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83585-1-RR

    Affinity and Kinetic Characterization of 83585-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83585-1-RR against Human LUC7L were performed. The affinity constant is 99.9 pM.

  • Featured Product
  • KD/KO验证

LUC7L Recombinant monoclonal antibody, PBS Only

LUC7L Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83585-1-PBS

产品说明书

CloneNo. 240311B10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

Putative RNA-binding protein Luc7-like 1, LUC7L1, LUC7B1, LUC7 LIKE, hLuc7B1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83585-1-PBS targets LUC7L in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9170

Product name: Recombinant human LUC7L protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-354 aa of BC065198

Sequence: MQMGDETRQRVKFTDDRVCKSHLLDCCPHDILAGTRMDLGECTKIHDLALRADYEIASKERDLFFELDAMDHLESFIAECDRRTELAKKRLAETQEEISAEVSAKAEKVHELNEEIGKLLAKAEQLGAEGNVDESQKILMEVEKVRAKKKEAEEEYRNSMPASSFQQQKLRVCEVCSAYLGLHDNDRRLADHFGGKLHLGFIQIREKLDQLRKTVAEKQEKRNQDRLRRREEREREERLSRRSGSRTRDRRRSRSRDRRRRRSRSTSRERRKLSRSRSRDRHRRHRSRSRSHSRGHRRASRDRSAKYKFSRERASREESWESGRSERGPPDWRLESSNGKMASRRSEEKEAGEI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 LUC7-like (S. cerevisiae)
别名 Putative RNA-binding protein Luc7-like 1, LUC7L1, LUC7B1, LUC7 LIKE, hLuc7B1
计算分子量 44 kDa
观测分子量38 kDa and 44 kDa
GenBank蛋白编号BC065198
基因名称 LUC7L
Gene ID (NCBI) 55692
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9NQ29
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LUC7L, also known as LUC7L1, belongs to the Luc7 family. LUC7L encodes a putative RNA binding protein similar to the yeast Luc7p subunit of the U1 snRNP splicing complex, which is normally required for 5` splice site selection (PMID: 15474286). LUC7L is widely expressed. LUC7L has 3 isoforms with the molecular mass of 38, 42 and 44 kDa.

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