验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse brain tissue |
| Positive IHC detected in | human hepatocirrhosis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20331-1-AP targets LTBR in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | LTBR fusion protein Ag14065 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | lymphotoxin beta receptor (TNFR superfamily, member 3) |
| 别名 | D12S370, LT BETA R, Lymphotoxin beta receptor, Lymphotoxin-beta receptor, TNF R III |
| 计算分子量 | 435 aa, 47 kDa |
| 观测分子量 | 47-60 kDa |
| GenBank蛋白编号 | BC026262 |
| 基因名称 | LTBR |
| Gene ID (NCBI) | 4055 |
| RRID | AB_10694276 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P36941 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LTBR antibody 20331-1-AP | Download protocol |
| IHC protocol for LTBR antibody 20331-1-AP | Download protocol |
| IF protocol for LTBR antibody 20331-1-AP | Download protocol |
| FC protocol for LTBR antibody 20331-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Ther Activation of NF-κB-Inducing Kinase in Islet β Cells Causes β Cell Failure and Diabetes. | ||
Am J Pathol Lymphotoxins Promote the Progression of Human Lymphatic Malformation by Enhancing Lymphatic Endothelial Cell Proliferation.
| ||
Mol Med Rep Lymphotoxin β receptor activation promotes mRNA expression of RelA and pro-inflammatory cytokines TNFα and IL-1β in bladder cancer cells. | ||
Theranostics Islet α-cell Inflammation Induced By NF-κB inducing kinase (NIK) Leads to Hypoglycemia, Pancreatitis, Growth Retardation, and Postnatal Death in Mice. | ||
J Reprod Immunol A LIGHT-HVEM/LTβR axis contributes to the fibrosis of intrauterine adhesion |
