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验证数据展示

  • WB analysis using 87378-1-RR (same clone as 87378-1-PBS)

    WB analysis using 87378-1-RR (same clone as 87378-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87378-1-RR (LSP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87378-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 87378-1-RR (same clone as 87378-1-PBS)

    IHC staining of human tonsillitis using 87378-1-RR (same clone as 87378-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 87378-1-RR (LSP1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87378-1-PBS in a different storage buffer formulation.

  • IF Staining of Daudi using 87378-1-RR (same clone as 87378-1-PBS)

    IF Staining of Daudi using 87378-1-RR (same clone as 87378-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Daudi cells using LSP1 antibody (87378-1-RR, Clone: 252179A7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87378-1-PBS in a different storage buffer formulation.

  • Affinity of 87378-1-RR

    Affinity of 87378-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87378-1-RR against Human LSP1 were performed. The affinity constant is 3.05 nM.

LSP1 Recombinant monoclonal antibody, PBS Only

LSP1 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 87378-1-PBS

产品说明书

CloneNo. 252179A7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

47 kDa actin-binding protein, Lymphocyte-specific antigen WP34, Lymphocyte-specific protein 1, pp52, WP34

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87378-1-PBS targets LSP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg5464

Product name: Recombinant human LSP1 protein

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 1-339 aa of NM_002339.2

Sequence: MAEASSDPGAEEREELLGPTAQWSVEDEEEAVHEQCQHERDRQLQAQDEEGGGHVPERPKQEMLLSLKPSEAPELDEDEGFGDWSQRPEQRQQHEGAQGALDSGEPPQCRSPEGEQEDRPGLHAYEKEDSDEVHLEELSLSKEGPGPEDTVQDNLGAAGAEEEQEEHQKCQQPRTPSPLVLEGTIEQSSPPLSPTTKLIDRTESLNRSIEKSNSVKKSQPDLPISKIDQWLEQYTQAIETAGRTPKLARQASIELPSMAVASTKSRWETGEVQAQSAAKTPSCKDIVAGDMSKKSLWEQKGGSKTSSTIKSTPSGKRYKFVATGHGKYEKVLVEGGPAP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 lymphocyte-specific protein 1
别名 47 kDa actin-binding protein, Lymphocyte-specific antigen WP34, Lymphocyte-specific protein 1, pp52, WP34
计算分子量37 kDa
观测分子量 52 kDa
GenBank蛋白编号NM_002339.2
基因名称 LSP1
Gene ID (NCBI) 4046
RRIDAB_3745497
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP33241-1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Lymphocyte-specific protein 1(LSP1), also known as leukocyte-specific protein 1, is an F-actin binding and cytoskeleton associated protein. The protein is expressed in lymphocytes, neutrophils, and macrophages. LSP1 is a substrate for mitogen-activated protein kinase (MAPK)-activated protein kinase 2 and protein kinase C. The implication of LSP1 in diverse biological processes, such as inflammation and malignancy, suggests the major regulatory role of LSP1 in signal transduction(PMID: 38254711).

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