验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, U-937 cells, Raji cells |
| Positive IP detected in | Raji cells |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15593-1-AP targets LSP1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag7944 Product name: Recombinant human LSP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-339 aa of BC001785 Sequence: MAEASSDPGAEEREELLGPTAQWSVEDEEEAVHEQCQHERDRQLQAQDEEGGGHVPERPKQEMLLSLKPSEAPELDEDEGFGDWSQRPEQRQQHEGAQGTLDSGEPPQCRSPEGEQEDRPGLHAYEKEDSDEVHLEELSLSKEGPGPEDTVQDNLGAAGAEEEQEEHQKCQQPRTPSPLVLEGTIEQSSPPLSPTTKLIDRTESLNRSIEKSNSVKKSQPDLPISKIDQWLEQYTQAIETAGRTPKLARQASIELPSMAVASTKSRWETGEVQAQSAAKTPSCKDIVAGDMSKKSLWEQKGGSKTSSTIKSTPSGKRYKFVATGHGKYEKVLVEGGPAP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | lymphocyte-specific protein 1 |
| 别名 | 47 kDa actin-binding protein, Lymphocyte-specific antigen WP34, Lymphocyte-specific protein 1, pp52, WP34 |
| 计算分子量 | 37 kDa |
| 观测分子量 | 52 kDa |
| GenBank蛋白编号 | BC001785 |
| 基因名称 | LSP1 |
| Gene ID (NCBI) | 4046 |
| RRID | AB_2250134 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P33241 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Lymphocyte-specific protein 1(LSP1), also known as leukocyte-specific protein 1, is an F-actin binding and cytoskeleton associated protein. The protein is expressed in lymphocytes, neutrophils, and macrophages. LSP1 is a substrate for mitogen-activated protein kinase (MAPK)-activated protein kinase 2 and protein kinase C. The implication of LSP1 in diverse biological processes, such as inflammation and malignancy, suggests the major regulatory role of LSP1 in signal transduction(PMID: 38254711).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for LSP1 antibody 15593-1-AP | Download protocol |
| IP protocol for LSP1 antibody 15593-1-AP | Download protocol |
| WB protocol for LSP1 antibody 15593-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
