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验证数据展示

  • WB analysis of HeLa using 10134-1-AP (same clone as 10134-1-PBS)

    WB analysis of HeLa using 10134-1-AP (same clone as 10134-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10134-1-AP (LSM8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10134-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 10134-1-AP (same clone as 10134-1-PBS)

    IHC staining of human kidney using 10134-1-AP (same clone as 10134-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10134-1-AP (LSM8 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 10134-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LSM8 antibody included
  • IHC staining of mouse colon using 10134-1-AP (same clone as 10134-1-PBS)

    IHC staining of mouse colon using 10134-1-AP (same clone as 10134-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10134-1-AP (LSM8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10134-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LSM8 antibody included
  • IHC staining of mouse colon using 10134-1-AP (same clone as 10134-1-PBS)

    IHC staining of mouse colon using 10134-1-AP (same clone as 10134-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10134-1-AP (LSM8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10134-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LSM8 antibody included
  • IF Staining of HeLa using 10134-1-AP (same clone as 10134-1-PBS)

    IF Staining of HeLa using 10134-1-AP (same clone as 10134-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LSM8 antibody (10134-1-AP) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10134-1-PBS in a different storage buffer formulation.

LSM8 Polyclonal antibody, PBS Only

LSM8 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 10134-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

NAA38, U6 snRNA-associated Sm-like protein LSm8, YJR022W

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy LSM8 Ready-To-Use IHC Kit. LSM8 primary antibody included.

产品信息

10134-1-PBS targets LSM8 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag0178

Product name: Recombinant human LSM8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-96 aa of BC002742

Sequence: MTSALENYINRTVAVITSDGRMIVGTLKGFDQTINLILDESHERVFSSSQGVEQVVLGLYIVRGDNVAVIGEIDEETDSALDLGNIRAEPLNSVAH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 LSM8 homolog, U6 small nuclear RNA associated (S. cerevisiae)
别名 NAA38, U6 snRNA-associated Sm-like protein LSm8, YJR022W
计算分子量 14 kDa /75 kDa
观测分子量 11 kDa
GenBank蛋白编号BC002742
基因名称 LSM8
Gene ID (NCBI) 51691
RRIDAB_513438
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95777
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family. Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing[PMID:15075370]. Lsm2-Lsm8 (Lsm stands for Like Sm), binds and stabilizes the 3′ end of the spliceosomal U6 snRNA Lsm proteins facilitate the annealing of U6 snRNA with U4 snRNA in vitro and interact with the U4/U6 annealing factor Prp24, suggesting that Lsm proteins function in U4/U6 snRNP formation in vivo [PMID:15485930, 17251193].

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