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LSM5 Polyclonal antibody

LSM5 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 10700-1-AP

Print datasheet

Synonyms

LSM5, YER146W

验证数据展示

  • WB analysis of HeLa using 10700-1-AP

    WB analysis of HeLa using 10700-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10700-1-AP (LSM5 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 10700-1-AP

    IF Staining of HeLa using 10700-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 10700-1-AP (LSM5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 10700-1-AP

    WB analysis using 10700-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10700-1-AP (LSM5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 10700-1-AP

    IHC staining of human liver cancer using 10700-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10700-1-AP (LSM5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LSM5 antibody included
  • IHC staining of human liver cancer using 10700-1-AP

    IHC staining of human liver cancer using 10700-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10700-1-AP (LSM5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LSM5 antibody included

经过测试的应用

Positive WB detected inHeLa cells, HL-60 cells, human brain tissue, human placenta tissue, mouse ovary tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy LSM5 Ready-To-Use IHC Kit. LSM5 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10700-1-AP targets LSM5 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human, mouse
Immunogen LSM5 fusion protein Ag1145 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name LSM5 homolog, U6 small nuclear RNA associated (S. cerevisiae)
Synonyms LSM5, YER146W
Calculated Molecular Weight 10 kDa
Observed Molecular Weight 10 kDa
GenBank Accession NumberBC005938
Gene Symbol LSM5
Gene ID (NCBI) 23658
RRIDAB_2138475
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y4Y9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LSM5 antibody 10700-1-APDownload protocol
IHC protocol for LSM5 antibody 10700-1-APDownload protocol
IF protocol for LSM5 antibody 10700-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols