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验证数据展示

  • WB analysis of HeLa using 10700-1-AP

    WB analysis of HeLa using 10700-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10700-1-AP (LSM5 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 10700-1-AP

    IF Staining of HeLa using 10700-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 10700-1-AP (LSM5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 10700-1-AP

    WB analysis using 10700-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10700-1-AP (LSM5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 10700-1-AP

    IHC staining of human liver cancer using 10700-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10700-1-AP (LSM5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LSM5 antibody included
  • IHC staining of human liver cancer using 10700-1-AP

    IHC staining of human liver cancer using 10700-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10700-1-AP (LSM5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LSM5 antibody included

LSM5 Polyclonal antibody

LSM5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 10700-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

YER146W, U6 snRNA-associated Sm-like protein LSm5

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HL-60 cells, human brain tissue, human placenta tissue, mouse ovary tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy LSM5 Ready-To-Use IHC Kit. LSM5 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10700-1-AP targets LSM5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag1145

Product name: Recombinant human LSM5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-91 aa of BC005938

Sequence: MAANATTNPSQLLPLELVDKCIGSRIHIVMKSDKEIVGTLLGFDDFVNMVLEDVTEFEITPEGRRITKLDQILLNGNNITMLVPGGEGPEV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 LSM5 homolog, U6 small nuclear RNA associated (S. cerevisiae)
别名 YER146W, U6 snRNA-associated Sm-like protein LSm5
计算分子量 10 kDa
观测分子量 10 kDa
GenBank蛋白编号BC005938
基因名称 LSM5
Gene ID (NCBI) 23658
RRIDAB_2138475
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y4Y9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for LSM5 antibody 10700-1-APDownload protocol
IHC protocol for LSM5 antibody 10700-1-APDownload protocol
WB protocol for LSM5 antibody 10700-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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