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LSM4 Polyclonal antibody

LSM4 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 10834-1-AP

Print datasheet

Synonyms

Glycine rich protein, GRP, LSM4, YER112W

验证数据展示

  • IHC staining of human lung cancer using 10834-1-AP

    IHC staining of human lung cancer using 10834-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10834-1-AP (LSM4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LSM4 antibody included
  • WB analysis of MCF-7 using 10834-1-AP

    WB analysis of MCF-7 using 10834-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 10834-1-AP (LSM4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 10834-1-AP

    IF Staining of MCF-7 using 10834-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 10834-1-AP (LSM4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inMCF7 cells, human placenta tissue, MDA-MB-231 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy LSM4 Ready-To-Use IHC Kit. LSM4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

10834-1-AP targets LSM4 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen LSM4 fusion protein Ag1280 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name LSM4 homolog, U6 small nuclear RNA associated (S. cerevisiae)
Synonyms Glycine rich protein, GRP, LSM4, YER112W
Calculated Molecular Weight 15 kDa
Observed Molecular Weight 15 kDa
GenBank Accession NumberBC022198
Gene Symbol LSM4
Gene ID (NCBI) 25804
RRIDAB_2138361
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y4Z0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sm-like proteins, such as LSM4, were identified in a variety of organisms based on sequence homology with the Sm protein family. Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing

实验方案

Product Specific Protocols
WB protocol for LSM4 antibody 10834-1-APDownload protocol
IHC protocol for LSM4 antibody 10834-1-APDownload protocol
IF protocol for LSM4 antibody 10834-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

EMBO Rep

EDC3 phosphorylation regulates growth and invasion through controlling P-body formation and dynamics.

Authors - Jeremiah J Bearss
View Article
humanWB

Mol Cell Biol

A quantitative high-throughput in vitro splicing assay identifies inhibitors of spliceosome catalysis.

Authors - Berg Michael G MG
View Article