验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, mouse liver tissue, pig heart tissue, mouse heart tissue, rat heart tissue |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human liver tissue, mouse heart tissue, mouse brown adipose tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26951-1-AP targets Perilipin 5 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat, pig |
| 文献引用反应性 | human, mouse, rat, pig, goat |
| 免疫原 |
CatNo: Ag25272 Product name: Recombinant human LSDP5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 381-463 aa of BC131524 Sequence: ILVERPEPLPDLADLVDEVIGGPDPRWAHLDWPAQQRAWEAEHRDGSGNGDGDRMGVAGDICEQEPETPSCPVKHTLMPELDF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | lipid storage droplet protein 5 |
| 别名 | LSDP5, OXPAT, PAT-1, Perilipin-5, PLIN5 |
| 计算分子量 | 463 aa, 51 kDa |
| 观测分子量 | 51-55 kDa |
| GenBank蛋白编号 | BC131524 |
| 基因名称 | Perilipin 5 |
| Gene ID (NCBI) | 440503 |
| RRID | AB_2880699 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q00G26 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Perilipin 5 (also known as LSDP5/OXPAT) is a lipid droplet (LD) coat protein that promotes association of LDs with mitochondria and is mainly present in tissues with a high fat-oxidative capacity, such as heart, skeletal muscles and brown adipose tissue. It plays an important role in the regulation of cardiac lipid storage and function.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Perilipin 5 antibody 26951-1-AP | Download protocol |
| IP protocol for Perilipin 5 antibody 26951-1-AP | Download protocol |
| WB protocol for Perilipin 5 antibody 26951-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Cell Multiomic integration reveals tumoral heterogeneity of lipid dependence within lethal group 3 medulloblastoma. | ||
Nat Metab Daytime-restricted feeding enhances running endurance without prior exercise in mice | ||
Nat Commun O-GlcNAc transferase plays dual antiviral roles by integrating innate immunity and lipid metabolism | ||
Metabolism Mitofusin-2 induced by exercise modifies lipid droplet-mitochondria communication, promoting fatty acid oxidation in male mice with NAFLD | ||
Metabolism Ubiquitination of cytoplasmic HMGB1 by RNF186 regulates hepatic lipophagy in non-alcoholic fatty liver disease | ||
Theranostics Chronic intermittent hypoxia increases Parkinson's disease susceptibility via PPARα-mediated lipid droplet-mitochondrial dysfunction. |
