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验证数据展示

  • WB analysis of Jurkat using 24666-1-AP (same clone as 24666-1-PBS)

    WB analysis of Jurkat using 24666-1-AP (same clone as 24666-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24666-1-AP (LRSAM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24666-1-PBS in a different storage buffer formulation.

  • IHC staining of human skeletal muscle using 24666-1-AP (same clone as 24666-1-PBS)

    IHC staining of human skeletal muscle using 24666-1-AP (same clone as 24666-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 24666-1-AP (LRSAM1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 24666-1-PBS in a different storage buffer formulation.

  • IHC staining of human skeletal muscle using 24666-1-AP (same clone as 24666-1-PBS)

    IHC staining of human skeletal muscle using 24666-1-AP (same clone as 24666-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 24666-1-AP (LRSAM1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 24666-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 24666-1-AP (same clone as 24666-1-PBS)

    IF Staining of A431 using 24666-1-AP (same clone as 24666-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using LRSAM1 antibody (24666-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 24666-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

LRSAM1 Polyclonal antibody, PBS Only

LRSAM1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 24666-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

RING-type E3 ubiquitin transferase LRSAM1, RIFLE, Leucine-rich repeat and sterile alpha motif-containing protein 1, hTAL, EC:2.3.2.27

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

24666-1-PBS targets LRSAM1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag20415

Product name: Recombinant human LRSAM1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 501-723 aa of BC009239

Sequence: QEMISEQRWALSSLLQQLLKEKQQREEELREILTELEAKSETRQENYWLIQYQRLLNQKPLSLKLQEEGMERQLVALLEELSAEHYLPIFAHHRLSLDLLSQMSPGDLAKVGVSEAGLQHEILRRVQELLDAARIQPELKPPMGEVVTPTAPQEPPESVRPSAPPAELEVQASECVVCLEREAQMIFLNCGHVCCCQQCCQPLRTCPLCRQDIAQRLRIYHSS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 leucine rich repeat and sterile alpha motif containing 1
别名 RING-type E3 ubiquitin transferase LRSAM1, RIFLE, Leucine-rich repeat and sterile alpha motif-containing protein 1, hTAL, EC:2.3.2.27
计算分子量 723 aa, 84 kDa
观测分子量 84 kDa
GenBank蛋白编号BC009239
基因名称 LRSAM1
Gene ID (NCBI) 90678
RRIDAB_2879665
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6UWE0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LRSAM1 also named as hTAL, RIFLE orTsg101 associated ligase is a 723 amino acid protein, which contains 6 LRR repeats, 1 Ring-type zinc finger and 1 SAM domain. LRSAM1 is localizes in cytoplasm and highly expressed in adult spinal cord motoneurons as well as in fetal spinal cord and muscle tissue. As a E3 ubiquitin protein ligase, LRSAM1 mediates monoubiquitination of TSG101 at multiple sites, leading to inactivate the ability of TSG101 to sort endocytic and exocytic cargos, LRSAM1 gene mutation has an association with Charcot Maire Tooth disease 2P.

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