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LRRTM1 Polyclonal antibody

LRRTM1 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 14288-1-AP

Print datasheet

Synonyms

LRRTM1

验证数据展示

  • WB analysis of HEK-293 using 14288-1-AP

    WB analysis of HEK-293 using 14288-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14288-1-AP (LRRTM1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 14288-1-AP

    IHC staining of human testis using 14288-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14288-1-AP (LRRTM1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 14288-1-AP

    IHC staining of human testis using 14288-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14288-1-AP (LRRTM1 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of mouse brain using 14288-1-AP

    IF Staining of mouse brain using 14288-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 14288-1-AP (LRRTM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, mouse brain tissue
Positive IHC detected inhuman testis tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14288-1-AP targets LRRTM1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen LRRTM1 fusion protein Ag5602 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name leucine rich repeat transmembrane neuronal 1
Synonyms LRRTM1
Calculated Molecular Weight 59 kDa
Observed Molecular Weight 59 kDa
GenBank Accession NumberBC045113
Gene Symbol LRRTM1
Gene ID (NCBI) 347730
RRIDAB_2138065
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86UE6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LRRTM1 antibody 14288-1-APDownload protocol
IHC protocol for LRRTM1 antibody 14288-1-APDownload protocol
IF protocol for LRRTM1 antibody 14288-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols