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验证数据展示

  • WB analysis using 86510-1-RR (same clone as 86510-1-PBS)

    WB analysis using 86510-1-RR (same clone as 86510-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86510-1-RR (LRRC8D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86510-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 86510-1-RR (same clone as 86510-1-PBS)

    IF Staining of HEK-293 using 86510-1-RR (same clone as 86510-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using LRRC8D antibody (86510-1-RR, Clone: 251257E6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86510-1-PBS in a different storage buffer formulation.

LRRC8D Recombinant monoclonal antibody, PBS Only

LRRC8D Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86510-1-PBS

产品说明书

CloneNo. 251257E6

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IF/ICC, Indirect ELISA

别名

HsLRRC8D, Leucine-rich repeat-containing protein 5, Leucine-rich repeat-containing protein 8D, LRRC5, Volume-regulated anion channel subunit LRRC8D

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86510-1-PBS targets LRRC8D in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag2138

Product name: Recombinant human LRRC8D protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 515-858 aa of BC024159

Sequence: LQELHLCHCPAKVEQTAFSFLRDHLRCLHVKFTDVAEIPAWVYLLKNLRELYLIGNLNSENNKMIGLESLRELRHLKILHVKSNLTKVPSNITDVAPHLTKLVIHNDGTKLLVLNSLKKMMNVAELELQNCELERIPHAIFSLSNLQELDLKSNNIRTIEEIISFQHLKRLTCLKLWHNKIVTIPPSITHVKNLESLYFSNNKLESLPVAVFSLQKLRCLDVSYNNISMIPIEIGLLQNLQHLHITGNKVDILPKQLFKCIKLRTLNLGQNCITSLPEKVGQLSQLTQLELKGNCLDRLPAQLGQCRMLKKSGLVVEDHLFDTLPLEVKEALNQDINIPFANGI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 leucine rich repeat containing 8 family, member D
别名 HsLRRC8D, Leucine-rich repeat-containing protein 5, Leucine-rich repeat-containing protein 8D, LRRC5, Volume-regulated anion channel subunit LRRC8D
计算分子量 858 aa, 98 kDa
观测分子量 98-120 kDa
GenBank蛋白编号BC024159
基因名称 LRRC8D
Gene ID (NCBI) 55144
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ7L1W4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LRRC8D (or LRRC5L) gene encodes a 98-kDa multi-pass membrane protein leucine-rich repeat-containing protein 8D, which contains 13 LRRs (leucine-rich repeats). The molecular function of this protein is so far not well understood. Recently, LRRC8D gene was found to be up-regulated after IL-2 deprivation in immortalised Interleukin-2(IL-2)-dependent human T cells, indicating a possible involvement of LRRC8D in cancer development. This LRRC8D antibody can't cross-recognize LRRC8A.

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