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  • Featured Product
  • KD/KO Validated

LRRC47 Polyclonal antibody

LRRC47 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 23217-1-AP

Print datasheet

Synonyms

KIAA1185, LRRC47

验证数据展示

  • WB analysis of mouse liver using 23217-1-AP

    WB analysis of mouse liver using 23217-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 23217-1-AP (LRRC47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 23217-1-AP

    IHC staining of human pancreas using 23217-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23217-1-AP (LRRC47 Antibody) at dilution of 1:50.

  • IHC staining of human pancreas using 23217-1-AP

    IHC staining of human pancreas using 23217-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23217-1-AP (LRRC47 Antibody) at dilution of 1:50.

  • IF Staining of L02 using 23217-1-AP

    IF Staining of L02 using 23217-1-AP

    Immunofluorescent analysis of L02 cells using 23217-1-AP (LRRC47 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse liver tissue, L02 cells, Neuro-2a cells, NIH/3T3 cells, rat liver tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inL02 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 23217-1-AP is LRRC47 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB, IHC, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen LRRC47 fusion protein Ag19736 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name leucine rich repeat containing 47
Synonyms KIAA1185, LRRC47
Calculated Molecular Weight 583 aa, 63 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC031301
Gene Symbol LRRC47
Gene ID (NCBI) 57470
RRIDAB_2879234
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ8N1G4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LRRC47 antibody 23217-1-APDownload protocol
IHC protocol for LRRC47 antibody 23217-1-APDownload protocol
IF protocol for LRRC47 antibody 23217-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

Structural basis for the final steps of human 40S ribosome maturation.

Authors - Michael Ameismeier
  • KD Validated
View Article
humanWB

bioRxiv

LARP1 senses free ribosomes to coordinate supply and demand of ribosomal proteins

Authors - James A Saba
  • KD Validated
View Article
humanWB,CoIP,IHC

Cell Rep

The C9ORF72 repeat expansion alters neurodevelopment

Authors - Eric Hendricks
View Article