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验证数据展示

  • IHC staining of human testis using 20457-1-AP

    IHC staining of human testis using 20457-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20457-1-AP (LRRC41 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with LRRC41 antibody included
  • WB analysis using 20457-1-AP

    WB analysis using 20457-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20457-1-AP (LRRC41 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 20457-1-AP

    IP experiment of K-562 using 20457-1-AP

    IP result of anti-LRRC41 (IP:20457-1-AP, 4ug; Detection:20457-1-AP 1:300) with K-562 cells lysate 1820 ug.

LRRC41 Polyclonal antibody

LRRC41 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 20457-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

Protein Muf1, PP7759, MUF1, Leucine-rich repeat-containing protein 41

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inH9C2 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman testis tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy LRRC41 Ready-To-Use IHC Kit. LRRC41 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20457-1-AP targets LRRC41 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag14266

Product name: Recombinant human LRRC41 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 223-572 aa of BC004953

Sequence: STLELFTVPLSTEAALTLCHLLSSWVSLESLTLSYNGLGSNIFRLLDSLRALSGQAGCRLRALHLSDLFSPLPILELTRAIVRALPLLRVLSIRVDHPSQRDNPGVPGNAGPPSHIIGDEEIPENCLEQLEMGFPRGAQPAPLLCSVLKASGSLQQLSLDSATFASPQDFGLVLQTLKEYNLALKRLSFHDMNLADCQSEVLFLLQNLTLQEITFSFCRLFEKRPAQFLPEMVAAMKGNSTLKGLRLPGNRLGNAGLLALADVFSEDSSSSLCQLDISSNCIKPDGLLEFAKRLERWGRGAFGHLRLFQNWLDQDAVTAREAIRRLRATCHVVSDSWDSSQAFADYVSTM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 leucine rich repeat containing 41
别名 Protein Muf1, PP7759, MUF1, Leucine-rich repeat-containing protein 41
计算分子量 812 aa, 87 kDa
观测分子量 80-90 kDa
GenBank蛋白编号BC004953
基因名称 LRRC41
Gene ID (NCBI) 10489
RRIDAB_10858385
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15345
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for LRRC41 antibody 20457-1-APDownload protocol
IP protocol for LRRC41 antibody 20457-1-APDownload protocol
WB protocol for LRRC41 antibody 20457-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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