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验证数据展示

  • WB analysis using 28057-1-AP

    WB analysis using 28057-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28057-1-AP (LRRC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 28057-1-AP

    IHC staining of mouse skeletal muscle using 28057-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 28057-1-AP (LRRC2 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

LRRC2 Polyclonal antibody

LRRC2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28057-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat, mouse

应用

WB, IHC, ELISA

别名

LRRC2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28057-1-AP targets LRRC2 in WB, IHC, ELISA applications and shows reactivity with mouse, rat, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse, rat, mouse
免疫原 LRRC2 fusion protein Ag27830 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 leucine rich repeat containing 2
别名 LRRC2
计算分子量 371 aa, 43 kDa
观测分子量38 kDa
GenBank蛋白编号BC029118
基因名称 LRRC2
Gene ID (NCBI) 79442
RRIDAB_3669639
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BYS8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Leucine Rich Repeat Containing 2 (LRRC2) was found to be localized to the mitochondria in human cells and transcriptionally regulated by the mitochondrial master regulator Pgc-1α. It has been reported that Lrrc2 transcript abundance correlates with that of β-MHC, a canonical marker of cardiac hypertrophy in humans, and experimentally demonstrated an elevation in Lrrc2 transcript in vitro and in vivo rodent models of cardiac hypertrophy as well as in patients with dilated cardiomyopathy. (PMID: 28158196)

实验方案

Product Specific Protocols
WB protocol for LRRC2 antibody 28057-1-APDownload protocol
IHC protocol for LRRC2 antibody 28057-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols