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验证数据展示

  • WB analysis of mouse testis using 24571-1-AP

    WB analysis of mouse testis using 24571-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 24571-1-AP (LRRC18 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 24571-1-AP

    IP experiment of mouse testis using 24571-1-AP

    IP result of anti-LRRC18 (IP:24571-1-AP, 3ug; Detection:24571-1-AP 1:500) with mouse testis tissue lysate 4000ug.

  • IHC staining of human testis using 24571-1-AP

    IHC staining of human testis using 24571-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24571-1-AP (LRRC18 Antibody) at dilution of 1:50 (under 40x lens).

LRRC18 Polyclonal antibody

LRRC18 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 24571-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, IHC, ELISA

别名

LRRC18, UNQ933, UNQ9338, VKGE9338

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24571-1-AP targets LRRC18 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag20996

Product name: Recombinant human LRRC18 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-261 aa of BC036722

Sequence: MVKGEKGPKGKKITLKVARNCIKITFDGKKRLDLSKMGITTFPKCILRLSDMDELDLSRNLIRKIPDSISKFQNLRWLDLHSNYIDKLPESIGQMTSLLYLNVSNNRLTSNGLPVELKQLKNIRAVNLGLNHLDSVSTTLGALKELHEVGLHDNLLNNIPVSISKLPKLKKLNIKRNPFPKPGESEIFIDSIRRLENLYVVEEKDLCAACLRKCQNARDNLNRIKNMATTTPRKTIFPNLISPNSMAKDSWEDWRIRLTSS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 leucine rich repeat containing 18
别名 LRRC18, UNQ933, UNQ9338, VKGE9338
计算分子量 261 aa, 30 kDa
观测分子量 30 kDa
GenBank蛋白编号BC036722
基因名称 LRRC18
Gene ID (NCBI) 474354
RRIDAB_2879614
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N456
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Leucine rich repeat containing protein 18 ( LRRC18 ) encodes a 261 amino-acids protein (29.6 kDa), which contains 7 leucine rich repeat and belongs to LRR superfamily. The function of protein is still non-known, but it may be involved in the regulation of spermatogenesis and sperm maturation. The protein localizes in cytoplasm and express in some male reproduction tissues.

实验方案

Product Specific Protocols
IHC protocol for LRRC18 antibody 24571-1-APDownload protocol
IP protocol for LRRC18 antibody 24571-1-APDownload protocol
WB protocol for LRRC18 antibody 24571-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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