×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 21175-1-AP

    WB analysis using 21175-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 21175-1-AP

    IF Staining of U2OS using 21175-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 21175-1-AP (LRPPRC antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). F-actin is stained using CL555-phalloidin (red) and DNA is stained by DAPI (blue).

  • IF Staining of HeLa using 21175-1-AP

    IF Staining of HeLa using 21175-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LRPPRC antibody (21175-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • IHC staining of human kidney using 21175-1-AP

    IHC staining of human kidney using 21175-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21175-1-AP (LRPPRC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LRPPRC antibody included
  • IHC staining of human kidney using 21175-1-AP

    IHC staining of human kidney using 21175-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21175-1-AP (LRPPRC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LRPPRC antibody included
  • IHC staining of human colon using 21175-1-AP

    IHC staining of human colon using 21175-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 21175-1-AP (LRPPRC antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LRPPRC antibody included
  • WB analysis using 21175-1-AP

    WB analysis using 21175-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21175-1-AP (LRPPRC antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 21175-1-AP

    IHC staining of human colon using 21175-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 21175-1-AP (LRPPRC antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LRPPRC antibody included
  • IP experiment of HeLa using 21175-1-AP

    IP experiment of HeLa using 21175-1-AP

    IP result of anti-LRPPRC (IP:21175-1-AP, 5ug; Detection:21175-1-AP 1:1000) with HeLa cells lysate 1200ug.

  • IHC staining of mouse heart using 21175-1-AP

    IHC staining of mouse heart using 21175-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21175-1-AP (LRPPRC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LRPPRC antibody included
  • Featured Product
  • KD/KO Validated

LRPPRC Polyclonal antibody

LRPPRC Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 21175-1-AP

产品说明书

引用文献(58)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (3)

别名

130 kDa leucine-rich protein, GP130, Leucine-rich PPR motif-containing protein, mitochondrial, LRP 130, LRP130

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHCT 116 cells, mouse heart tissue, HepG2 cells, mouse skeletal muscle tissue, mouse kidney tissue, mouse liver tissue, HeLa cells, rat kidney tissue, rat liver tissue, rat heart tissue, PC-12 cells, SH-SY5Y cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human colon tissue, human heart tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells, HepG2 cells, HeLa cells, HEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy LRPPRC Ready-To-Use IHC Kit. LRPPRC primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21175-1-AP targets LRPPRC in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, RIP, ELISA
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原

CatNo: Ag15452

Product name: Recombinant human LRPPRC protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1014-1394 aa of BC050311

Sequence: LWYEDEKHSLNSSSASTTEPDFQKDILIACRLNQKKGAYDIFLNAKEQNIVFNAETYSNLIKLLMSEDYFTQAMEVKAFAETHIKGFTLNDAANSRLIITQVRRDYLKEAVTTLKTVLDQQQTPSRLAVTRVIQALAMKGDVENIEVVQKMLNGLEDSIGLSKMVFINNIALAQIKNNNIDAAIENIENMLTSENKVIEPQYFGLAYLFRKVIEEQLEPAVEKISIMAERLANQFAIYKPVTDFFLQLVDAGKVDDARALLQRCGAIAEQTPILLLFLLRNSRKQGKASTVKSVLELIPELNEKEEAYNSLMKSYVSEKDVTSAKALYEHLTAKNTKLDDLFLKRYASLLKYAGEPVPFIEPPESFEFYAQQLRKLRENSS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 leucine-rich PPR-motif containing
别名 130 kDa leucine-rich protein, GP130, Leucine-rich PPR motif-containing protein, mitochondrial, LRP 130, LRP130
计算分子量 1394 aa, 158 kDa
观测分子量 130 kDa
GenBank蛋白编号BC050311
基因名称 LRPPRC
Gene ID (NCBI) 10128
RRIDAB_10733879
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP42704
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LRPPRC (also called LRP130 or GP130) is a 130-kDa RNA-binding protein of the pentatricopeptide repeat family. LRPPRC localizes primarily to the mitochondria where it binds to poly(A) mRNA. It plays a role in the translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. LRPPRC has also been shown to regulate nuclear gene transcription and to bind specific RNA molecules in both the nucleus and the cytoplasm. Mutations in the LRPPRC gene are associated with the French-Canadian type of Leigh syndrome.

实验方案

Product Specific Protocols
IF protocol for LRPPRC antibody 21175-1-APDownload protocol
IHC protocol for LRPPRC antibody 21175-1-APDownload protocol
IP protocol for LRPPRC antibody 21175-1-APDownload protocol
WB protocol for LRPPRC antibody 21175-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell

QKI shuttles internal m7G-modified transcripts into stress granules and modulates mRNA metabolism

Authors - Zhicong Zhao
View Article
mouseIP

Nat Biotechnol

Translation efficiency of mRNAs is increased by antisense oligonucleotides targeting upstream open reading frames.

Authors - Xue-Hai Liang
View Article
humanWB,CoIP

Autophagy

A/(H1N1) pdm09 NS1 promotes viral replication by enhancing autophagy through hijacking the IAV negative regulatory factor LRPPRC

Authors - Xing Guo
View Article
humanWB,IF

Nat Commun

A mosquito salivary protein promotes flavivirus transmission by activation of autophagy.

Authors - Peng Sun
View Article
mouseWB,IF,CoIP

Nat Commun

Mitochondrial membrane proteins and VPS35 orchestrate selective removal of mtDNA

Authors - Ayesha Sen
View Article
IF

Nat Commun

Targeted therapies of inflammatory diseases with intracellularly gelated macrophages in mice and rats

Authors - Cheng Gao
View Article
||
New chat

Able™

正在加载，请稍候...