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- KD/KO Validated
PTPRA Polyclonal antibody
PTPRA Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse
Applications
IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, rat liver tissue, MCF-7 cells, SKOV-3 cells |
| Positive IP detected in | A431 cells |
| Positive IHC detected in | human lung cancer tissue, human urothelial carcinoma tissue, mouse heart tissue, mouse kidney tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | A431 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13079-1-AP targets PTPRA in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.
| Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | WB, IP, IF |
| Tested Reactivity | human, rat, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | PTPRA fusion protein Ag3760 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | protein tyrosine phosphatase, receptor type, A |
| Synonyms | HEPTP, HLPR, HPTPA, HPTPalpha, LRP, PTPA, PTPRA, PTPRL2, R PTP alpha, RPTPA |
| Calculated Molecular Weight | 793 aa, 91 kDa |
| Observed Molecular Weight | 90-105 kDa, 140 kDa |
| GenBank Accession Number | BC027308 |
| Gene Symbol | PTPRA |
| Gene ID (NCBI) | 5786 |
| RRID | AB_2269055 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P18433 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PTPRA antibody 13079-1-AP | Download protocol |
| IHC protocol for PTPRA antibody 13079-1-AP | Download protocol |
| IF protocol for PTPRA antibody 13079-1-AP | Download protocol |
| IP protocol for PTPRA antibody 13079-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncol Lett PTPRA facilitates cancer growth and migration via the TNF-α-mediated PTPRA-NF-κB pathway in MCF-7 breast cancer cells.
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Oncogene Glucose-mediated N-glycosylation of RPTPα affects its subcellular localization and Src activation | ||
Sci Signal Clustering of phosphatase RPTPα promotes Src signaling and the arthritogenic action of synovial fibroblasts | ||
EBioMedicine The circRNA circPTPRA suppresses epithelial-mesenchymal transitioning and metastasis of NSCLC cells by sponging miR-96-5p.
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