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验证数据展示

  • WB analysis using 33107-1-AP

    WB analysis using 33107-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 33107-1-AP (LRIG3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 33107-1-AP

    IF Staining of U2OS using 33107-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using LRIG3 antibody (33107-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

LRIG3 Polyclonal antibody

LRIG3 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 33107-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

KIAA3016, LIG 3, LIG3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, U-251 cells, U-87 MG cells
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

33107-1-AP targets LRIG3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg2853

Product name: Recombinant Human LRIG3 protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 28-807 aa of NM_153377.4

Sequence: DSGGRGELGQPSGVAAERPCPTTCRCLGDLLDCSRKRLARLPEPLPSWVARLDLSHNRLSFIKASSMSHLQSLREVKLNNNELETIPNLGPVSANITLLSLAGNRIVEILPEHLKEFQSLETLDLSSNNISELQTAFPALQLKYLYLNSNRVTSMEPGYFDNLANTLLVLKLNRNRISAIPPKMFKLPQLQHLELNRNKIKNVDGLTFQGLGALKSLKMQRNGVTKLMDGAFWGLSNMEILQLDHNNLTEITKGWLYGLLMLQELHLSQNAINRISPDAWEFCQKLSELDLTFNHLSRLDDSSFLGLSLLNTLHIGNNRVSYIADCAFRGLSSLKTLDLKNNEISWTIEDMNGAFSGLDKLRRLILQGNRIRSITKKAFTGLDALEHLDLSDNAIMSLQGNAFSQMKKLQQLHLNTSSLLCDCQLKWLPQWVAENNFQSFVNASCAHPQLLKGRSIFAVSPDGFVCDDFPKPQITVQPETQSAIKGSNLSFICSAASSSDSPMTFAWKKDNELLHDAEMENYAHLRAQGGEVMEYTTILRLREVEFASEGKYQCVISNHFGSSYSVKAKLTVNMLPSFTKTPMDLTIRAGAMARLECAAVGHPAPQIAWQKDGGTDFPAARERRMHVMPEDDVFFIVDVKIEDIGVYSCTAQNSAGSISANATLTVLETPSFLRPLLDRTVTKGETAVLQCIAGGSPPPKLNWTKDDSPLVVTERHFFAAGNQLLIIVDSDVSDAGKYTCEMSNTLGTERGNVRLSVIPTPTCDSPQMTAPSLDDDGWAT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 leucine-rich repeats and immunoglobulin-like domains 3
别名 KIAA3016, LIG 3, LIG3
计算分子量123 kDa
观测分子量117 kDa
GenBank蛋白编号NM_153377.4
基因名称 LRIG3
Gene ID (NCBI) 121227
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ6UXM1-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LRIG3 is a member of the LRIG family of transmembrane proteins, characterized by leucine-rich repeats and immunoglobulin-like domains. It plays a crucial role in regulating various signaling pathways, including those involved in cell proliferation, differentiation, and immune responses.

实验方案

Product Specific Protocols
IF protocol for LRIG3 antibody 33107-1-APDownload protocol
WB protocol for LRIG3 antibody 33107-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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