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验证数据展示

  • WB analysis using 83880-5-RR

    WB analysis using 83880-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83880-5-RR (LRFN5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 83880-5-RR

    IHC staining of mouse brain using 83880-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83880-5-RR (LRFN5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83880-5-RR

    Affinity and Kinetic Characterization of 83880-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83880-5-RR against Human LRFN5 were performed. The affinity constant is 2.95 nM.

LRFN5 Recombinant monoclonal antibody

LRFN5 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 83880-5-RR

产品说明书

CloneNo. 241060F5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

241060F5, C14orf146, FIGLER8, Leucine-rich repeat and fibronectin type-III domain-containing protein 5, SALM5

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse heart tissue, rat heart tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83880-5-RR targets LRFN5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag22152

Product name: Recombinant human LRFN5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 579-719 aa of BC043165

Sequence: QGCSVTLPQSVSKQAVGHEENAQCCKATSDNVIQSSETCSSQDSSTTTSALPPSWTSSTSVSQKQKRKTGTKPSTEPQNEAVTNVESQNTNRNNSTALQLASRPPDSVTEGPTSKRAHIKPNALLTNVDQIVQETQRLELI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 leucine rich repeat and fibronectin type III domain containing 5
别名 241060F5, C14orf146, FIGLER8, Leucine-rich repeat and fibronectin type-III domain-containing protein 5, SALM5
计算分子量 719 aa, 79 kDa
观测分子量79 kDa
GenBank蛋白编号BC043165
基因名称 LRFN5
Gene ID (NCBI) 145581
RRIDAB_3671463
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ96NI6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Leucine-rich repeat and fibronectin type-III domain-containing protein 5(LRFN5), belongs to a family of five small transmembrane protein genes involved in the development, organization, and plasticity of synapses. LRFN5 has another synonym which stands for synaptic adhesion-like molecule 5 (SALM5). SALMs are newly characterized adhesion molecules predominantly expressed in the brain contributing to neurite outgrowth and synapse formation. (PMID: 37280213)

实验方案

Product Specific Protocols
IHC protocol for LRFN5 antibody 83880-5-RRDownload protocol
WB protocol for LRFN5 antibody 83880-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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