LRAT Recombinant antibody

LRAT Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85628-1-RR

产品说明书

CloneNo. 243058B5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

EC:2.3.1.135, Lecithin retinol acyltransferase, Phosphatidylcholine--retinol O-acyltransferase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Planning an IHC experiment? We recommend our IHCeasy LRAT Ready-To-Use IHC Kit. LRAT primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85628-1-RR targets LRAT in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 LRAT fusion protein Ag3556 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 lecithin retinol acyltransferase (phosphatidylcholine--retinol O-acyltransferase)
别名 EC:2.3.1.135, Lecithin retinol acyltransferase, Phosphatidylcholine--retinol O-acyltransferase
计算分子量 230 aa, 26 kDa
观测分子量 26 kDa, 50-54 kDa
GenBank蛋白编号BC031053
基因名称 LRAT
Gene ID (NCBI) 9227
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO95237
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LRAT belongs to the H-rev107 family. LRAT transfers the acyl group from the sn-1 position of phosphatidylcholine to all-trans retinol, producing all-trans retinyl esters. LRAT plays a critical role in vision. It provides the all-trans retinyl ester substrates for the isomerohydrolase which processes the esters into 11-cis-retinol in the retinal pigment epithelium; due to a membrane-associated alcohol dehydrogenase, LRAT monomers (26kDa) interact in membranes and form functional homodimers (50-54 kDa) through protein-protein interactions and disulfide bond formation.Defects in LRAT are a cause of severe early-onset retinal dystrophy (RD). This antibody was generated against the internal residues of human LRAT. It is predicted to detect endogenous LRAT.

实验方案

Product Specific Protocols
WB protocol for LRAT antibody 85628-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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