验证数据展示
经过测试的应用
Positive WB detected in | mouse testis tissue, rat testis tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:6000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
85628-1-RR targets LRAT in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | LRAT fusion protein Ag3556 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | lecithin retinol acyltransferase (phosphatidylcholine--retinol O-acyltransferase) |
别名 | EC:2.3.1.135, Lecithin retinol acyltransferase, Phosphatidylcholine--retinol O-acyltransferase |
计算分子量 | 230 aa, 26 kDa |
观测分子量 | 26 kDa, 50-54 kDa |
GenBank蛋白编号 | BC031053 |
基因名称 | LRAT |
Gene ID (NCBI) | 9227 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | O95237 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LRAT belongs to the H-rev107 family. LRAT transfers the acyl group from the sn-1 position of phosphatidylcholine to all-trans retinol, producing all-trans retinyl esters. LRAT plays a critical role in vision. It provides the all-trans retinyl ester substrates for the isomerohydrolase which processes the esters into 11-cis-retinol in the retinal pigment epithelium; due to a membrane-associated alcohol dehydrogenase, LRAT monomers (26kDa) interact in membranes and form functional homodimers (50-54 kDa) through protein-protein interactions and disulfide bond formation.Defects in LRAT are a cause of severe early-onset retinal dystrophy (RD). This antibody was generated against the internal residues of human LRAT. It is predicted to detect endogenous LRAT.
实验方案
Product Specific Protocols | |
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WB protocol for LRAT antibody 85628-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |