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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83657-5-RR (same clone as 83657-5-PBS)

    WB analysis using 83657-5-RR (same clone as 83657-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83657-5-RR (LPXN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83657-5-PBS in a different storage buffer formulation.

  • IF Staining of PC-3 using 83657-5-RR (same clone as 83657-5-PBS)

    IF Staining of PC-3 using 83657-5-RR (same clone as 83657-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using LPXN antibody (83657-5-RR, Clone: 240483G10 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83657-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83657-5-RR

    Affinity and Kinetic Characterization of 83657-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83657-5-RR against Human LPXN were performed. The affinity constant is 0.187 nM.

LPXN Recombinant antibody, PBS Only

LPXN Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83657-5-PBS

产品说明书

CloneNo. 240483G10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

LDPL, LDLP, 240483G10

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy LPXN Ready-To-Use IHC Kit. LPXN primary antibody included.

产品信息

83657-5-PBS targets LPXN in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 LPXN fusion protein Ag34956 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 leupaxin
别名 LDPL, LDLP, 240483G10
计算分子量 43 kDa
观测分子量 45 kDa
GenBank蛋白编号BC019035
基因名称 LPXN
Gene ID (NCBI) 9404
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO60711
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LPXN, also known as Leupaxin, LDPL, belongs to the paxillin family. LPXN Contributes to the regulation of cell adhesion, spreading, and cell migration and acts as a negative regulator in integrin-mediated cell adhesion events (PMID: 20543562). LPXN has four leucine-rich LD-motifs in the N-terminus and four LIM domains in the C-terminus. It may function in cell type-specific signaling by associating with PYK2, a member of the focal adhesion kinase family (PMID: 9565592). Studies in breast cancer implicate LPXN is involved in the regulation of ER action as a co-factor (PMID: 25955236). LPXN is expressed in prostate cancer cells and its expression intensity is directly linked to PCa progression (PMID: 18451096).