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LPXN Polyclonal antibody

LPXN Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 11307-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IF/ICC, IP, ELISA and More (2)

LDPL, LDLP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inDU 145 cells, PC-3 cells
Positive IP detected inPC-3 cells
Positive IF/ICC detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy LPXN Ready-To-Use IHC Kit. LPXN primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11307-1-AP targets LPXN in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用IHC, IF
经测试反应性 human
文献引用反应性mouse
免疫原 LPXN fusion protein Ag1845 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 leupaxin
别名 LDPL, LDLP
计算分子量 43 kDa
观测分子量 45 kDa
GenBank蛋白编号BC019035
基因名称 LPXN
Gene ID (NCBI) 9404
RRIDAB_2877756
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60711
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LPXN, also known as Leupaxin, LDPL, belongs to the paxillin family. LPXN Contributes to the regulation of cell adhesion, spreading, and cell migration and acts as a negative regulator in integrin-mediated cell adhesion events (PMID: 20543562). LPXN has four leucine-rich LD-motifs in the N-terminus and four LIM domains in the C-terminus. It may function in cell type-specific signaling by associating with PYK2, a member of the focal adhesion kinase family (PMID: 9565592). Studies in breast cancer implicate LPXN is involved in the regulation of ER action as a co-factor (PMID: 25955236). LPXN is expressed in prostate cancer cells and its expression intensity is directly linked to PCa progression (PMID: 18451096).

实验方案

Product Specific Protocols
WB protocol for LPXN antibody 11307-1-APDownload protocol
IF protocol for LPXN antibody 11307-1-APDownload protocol
IP protocol for LPXN antibody 11307-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

J Mol Cell Cardiol

Exogenous extracellular matrix proteins decrease cardiac fibroblast activation in stiffening microenvironment through CAPG.

Authors - Xinming Wang
mouseIHC

Mol Cell Biochem

STAT3 mediates ECM stiffness-dependent progression in ovarian cancer

Authors - Sun Chenchen
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