验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, HepG2 cells, mouse colon tissue, COLO 320 cells, rat brain tissue, human liver tissue |
| Positive IP detected in | mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
20045-1-AP targets LPHN3 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | latrophilin 3 |
| 别名 | CIRL 3, CIRL3, KIAA0768, latrophilin 3, LEC3, Lectomedin 3, LPHN3 |
| 计算分子量 | 162 kDa |
| 观测分子量 | 139 kDa |
| GenBank蛋白编号 | NM_015236 |
| 基因名称 | LPHN3 |
| Gene ID (NCBI) | 23284 |
| RRID | AB_10665552 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9HAR2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LPHN3, also named as KIAA0768 and LEC3, belongs to the G-protein coupled receptor 2 family and LN-TM7 subfamily. LPHN3 forms a heterodimer, consisting of a large extracellular region (p120) non-covalently linked to a seven-transmembrane moiety (p85). This antibody recognizes all the isoforms of LPHN3 and has no cross reaction with LPHN1 and LPHN2.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for LPHN3 antibody 20045-1-AP | Download protocol |
| IP protocol for LPHN3 antibody 20045-1-AP | Download protocol |
| WB protocol for LPHN3 antibody 20045-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
World J Biol Psychiatry Expression of the adult ADHD-associated gene ADGRL3 is dysregulated by risk variants and environmental risk factors. | ||
