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LPHN3 Polyclonal antibody

LPHN3 Polyclonal Antibody for FC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 20045-1-AP

Print datasheet

Synonyms

CIRL 3, CIRL3, KIAA0768, latrophilin 3, LEC3, Lectomedin 3, LPHN3

验证数据展示

  • WB analysis of mouse brain using 20045-1-AP

    WB analysis of mouse brain using 20045-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20045-1-AP (LPHN3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 20045-1-AP

    IP experiment of mouse brain using 20045-1-AP

    IP result of anti-LPHN3 (IP:20045-1-AP, 4ug; Detection:20045-1-AP 1:300) with mouse brain tissue lysate 4000ug.

  • FC experiment of HepG2 using 20045-1-AP

    FC experiment of HepG2 using 20045-1-AP

    1X10^6 HepG2 cells were stained with .2ug LPHN3 antibody (20045-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

经过测试的应用

Positive WB detected inmouse brain tissue, HepG2 cells, mouse colon tissue, COLO 320 cells, rat brain tissue, human liver tissue
Positive IP detected inmouse brain tissue
Positive FC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

20045-1-AP targets LPHN3 in WB, IP, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IP, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name latrophilin 3
Synonyms CIRL 3, CIRL3, KIAA0768, latrophilin 3, LEC3, Lectomedin 3, LPHN3
Calculated Molecular Weight 162 kDa
Observed Molecular Weight 139 kDa
GenBank Accession NumberNM_015236
Gene Symbol LPHN3
Gene ID (NCBI) 23284
RRIDAB_10665552
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HAR2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LPHN3, also named as KIAA0768 and LEC3, belongs to the G-protein coupled receptor 2 family and LN-TM7 subfamily. LPHN3 forms a heterodimer, consisting of a large extracellular region (p120) non-covalently linked to a seven-transmembrane moiety (p85). This antibody recognizes all the isoforms of LPHN3 and has no cross reaction with LPHN1 and LPHN2.

实验方案

Product Specific Protocols
WB protocol for LPHN3 antibody 20045-1-APDownload protocol
IP protocol for LPHN3 antibody 20045-1-APDownload protocol
FC protocol for LPHN3 antibody 20045-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

World J Biol Psychiatry

Expression of the adult ADHD-associated gene ADGRL3 is dysregulated by risk variants and environmental risk factors.

Authors - Rhiannon V McNeill
View Article