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验证数据展示

  • WB analysis using 15082-1-AP

    WB analysis using 15082-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15082-1-AP (LPCAT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 15082-1-AP

    IP experiment of HeLa using 15082-1-AP

    IP result of anti-LPCAT2 (IP:15082-1-AP, 4ug; Detection:15082-1-AP 1:5000) with HeLa cells lysate 1280 ug.

  • IF Staining of A431 using 15082-1-AP

    IF Staining of A431 using 15082-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using LPCAT2 antibody (15082-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IHC staining of mouse brain using 15082-1-AP

    IHC staining of mouse brain using 15082-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15082-1-AP (LPCAT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LPCAT2 antibody included
  • IHC staining of mouse brain using 15082-1-AP

    IHC staining of mouse brain using 15082-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15082-1-AP (LPCAT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LPCAT2 antibody included
  • IHC staining of rat thyroid gland using 15082-1-AP

    IHC staining of rat thyroid gland using 15082-1-AP

    Immunohistochemical analysis of paraffin-embedded rat thyroid gland tissue slide using 15082-1-AP (LPCAT2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LPCAT2 antibody included
  • IHC staining of rat thyroid gland using 15082-1-AP

    IHC staining of rat thyroid gland using 15082-1-AP

    Immunohistochemical analysis of paraffin-embedded rat thyroid gland tissue slide using 15082-1-AP (LPCAT2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LPCAT2 antibody included
  • IHC staining of mouse small intestine using 15082-1-AP

    IHC staining of mouse small intestine using 15082-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 15082-1-AP (LPCAT2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LPCAT2 antibody included
  • IHC staining of rat small intestine using 15082-1-AP

    IHC staining of rat small intestine using 15082-1-AP

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 15082-1-AP (LPCAT2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LPCAT2 antibody included
  • IHC staining of rat small intestine using 15082-1-AP

    IHC staining of rat small intestine using 15082-1-AP

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 15082-1-AP (LPCAT2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LPCAT2 antibody included
  • IHC staining of human rectal cancer using 15082-1-AP

    IHC staining of human rectal cancer using 15082-1-AP

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 15082-1-AP (LPCAT2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LPCAT2 antibody included
  • IHC staining of human rectal cancer using 15082-1-AP

    IHC staining of human rectal cancer using 15082-1-AP

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 15082-1-AP (LPCAT2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LPCAT2 antibody included

LPCAT2 Polyclonal antibody

LPCAT2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 15082-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

1-acylglycerol-3-phosphate O-acyltransferase 11, 1-acylglycerophosphocholine O-acyltransferase, 1-AGP acyltransferase 11, 1-AGPAT 11, 1-alkenylglycerophosphocholine O-acyltransferase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, LPS treated HEK-293, HeLa cells, Jurkat cells, RAW 264.7 cells
Positive IP detected inHeLa cells
Positive IHC detected inrat thyroid gland tissue, human rectal cancer tissue, mouse brain tissue, mouse small intestine tissue, rat small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy LPCAT2 Ready-To-Use IHC Kit. LPCAT2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15082-1-AP targets LPCAT2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 LPCAT2 fusion protein Ag7156 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lysophosphatidylcholine acyltransferase 2
别名 1-acylglycerol-3-phosphate O-acyltransferase 11, 1-acylglycerophosphocholine O-acyltransferase, 1-AGP acyltransferase 11, 1-AGPAT 11, 1-alkenylglycerophosphocholine O-acyltransferase
计算分子量 60 kDa
观测分子量 53 kDa
GenBank蛋白编号BC002472
基因名称 LPCAT2
Gene ID (NCBI) 54947
RRIDAB_2135557
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7L5N7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LPCAT2 antibody 15082-1-APDownload protocol
IHC protocol for LPCAT2 antibody 15082-1-APDownload protocol
IF protocol for LPCAT2 antibody 15082-1-APDownload protocol
IP protocol for LPCAT2 antibody 15082-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Antioxidants (Basel)

Mitochondrial Reactive Oxygen Species Formation Determines ACSL4/LPCAT2-Mediated Ferroptosis

Authors - Melanie Merkel
View Article