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验证数据展示

  • WB analysis of mouse liver using 26070-1-AP

    WB analysis of mouse liver using 26070-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 26070-1-AP (LOC123688 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

AGPHD1 Polyclonal antibody

AGPHD1 Polyclonal Antibody for WB, ELISA
Cat No. 26070-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

5-hydroxy-L-lysine kinase, AGPHD1, Hydroxylysine kinase, HYKK

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26070-1-AP targets AGPHD1 in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 AGPHD1 fusion protein Ag23420 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 similar to RIKEN cDNA C630028N24 gene
别名 5-hydroxy-L-lysine kinase, AGPHD1, Hydroxylysine kinase, HYKK
观测分子量 27-30 kDa
GenBank蛋白编号BC132753
基因名称 AGPHD1/HYKK
Gene ID (NCBI) 123688
RRIDAB_2880361
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDA2RU49
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AGPHD1 encodes the 5-hydroxy-L-lysine kinase which catalyzes the GTP-dependent phosphorylation of 5-hydroxy-L-lysine. It is primarily present in liver and kidneys, suggesting its major role in degrading 5Hyl derived from food. Its presence at lower levels in other tissues indicates that it participates also in the catabolism of 5Hyl derived from local collagen breakdown.

实验方案

Product Specific Protocols
WB protocol for AGPHD1 antibody 26070-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols