- Featured Product
- KD/KO Validated
LMX1B Polyclonal antibody
LMX1B Polyclonal Antibody for IF, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF, WB, ELISA and More (3)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
| Positive IF detected in | embryonic kidney tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18278-1-AP targets LMX1B in WB, IP, IF, IHC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF, WB, ELISA |
| Cited Applications | WB, IP, IF, IHC, ChIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | LMX1B fusion protein Ag13096 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | LIM homeobox transcription factor 1, beta |
| Synonyms | LIM/homeobox protein 1.2, LIM/homeobox protein LMX1B, LMX 1.2, LMX1.2, LMX1B |
| Calculated Molecular Weight | 372 aa, 42 kDa |
| Observed Molecular Weight | 42-45 kDa |
| GenBank Accession Number | BC112120 |
| Gene Symbol | LMX1B |
| Gene ID (NCBI) | 4010 |
| RRID | AB_11232226 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60663 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LMX1B, LIM homeodomain factor 1b, functions as a transcription factor, and is essential for the normal development of dorsal limb structures, the glomerular basement membrane, the anterior segment of the eye, and dopaminergic and serotonergic neurons. Lmx1b is a continuously expressed, terminal selector-type factor in 5-HT neurons. It is required for maintenance of central serotonergic neurons (PMID: 17151281, PMID: 14602809). It is expressed in brain, testis, skeletal muscle, and pancreatic islets (PMID: 35417751, PMID: 18512225).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LMX1B antibody 18278-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Autophagy A monolayer hiPSC culture system for autophagy/mitophagy studies in human dopaminergic neurons. | ||
Sci Signal Ubiquitination and functional modification of GluN2B subunit-containing NMDA receptors by Cbl-b in the spinal cord dorsal horn. | ||
Neurobiol Dis Maternal Herpesviridae infection during pregnancy alters midbrain dopaminergic signatures in adult offspring. | ||
Biomed Pharmacother LMX1B involved in the radioresistance, proliferation and migration of esophageal cancer cells.
| ||
Methods Mol Biol Imaging Autophagy in hiPSC-Derived Midbrain Dopaminergic Neuronal Cultures for Parkinson's Disease Research. | ||
J Cell Biol ATG8-dependent LMX1B-autophagy crosstalk shapes human midbrain dopaminergic neuronal resilience
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