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LMO7 Polyclonal antibody

LMO7 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 29392-1-AP

Print datasheet

Synonyms

F box only protein 20, FBX20, FBXO20, KIAA0858, LIM domain 7, LIM domain only protein 7, LMO 7, LMO7, LOMP

验证数据展示

  • WB analysis using 29392-1-AP

    WB analysis using 29392-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29392-1-AP (LMO7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 29392-1-AP

    IHC staining of mouse heart using 29392-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 29392-1-AP (LMO7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 29392-1-AP

    IHC staining of mouse heart using 29392-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 29392-1-AP (LMO7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 29392-1-AP

    IHC staining of mouse skeletal muscle using 29392-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 29392-1-AP (LMO7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 29392-1-AP

    IHC staining of mouse skeletal muscle using 29392-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 29392-1-AP (LMO7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, mouse lung tissue
Positive IHC detected inmouse heart tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29392-1-AP targets LMO7 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, mouse
Immunogen LMO7 fusion protein Ag30016 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name LIM domain 7
Synonyms F box only protein 20, FBX20, FBXO20, KIAA0858, LIM domain 7, LIM domain only protein 7, LMO 7, LMO7, LOMP
Calculated Molecular Weight 193 kDa
Observed Molecular Weight 140-160 kDa
GenBank Accession NumberNM_005358
Gene Symbol LMO7
Gene ID (NCBI) 4008
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WWI1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LMO7, also known as LOMP, FBX20, belongs to the single Calponin-homology (CH) domain-containing protein family, with a single actin-binding CH domain at the amino terminal end(PMID: 19459066). LMO7 also contains a PDZ domain and a carboxyl-terminal LIM domain, both of which have been described as protein-protein interacting domains(PMID: 15140894). LMO7 encodes three splice variants of calculated molecular weights of 196 kDa (P200), 157 kDa (P150), and 100 kDa (P100)(PMID: 26157009).

实验方案

Product Specific Protocols
WB protocol for LMO7 antibody 29392-1-APDownload protocol
IHC protocol for LMO7 antibody 29392-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols