验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, Jurkat cells, Raji cells, mouse brain tissue |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human lung cancer tissue, human colon tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Caco-2 cells, SH-SY5Y cells, HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10895-1-AP targets Lamin B2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag1335 Product name: Recombinant human LMNB2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 241-600 aa of BC006551 Sequence: EELRSQHDEQVRLYKLELEQTYQAKLDSAKLSSDQNDKAASAAREELKEARMRLESLSYQLSGLQKQASAAEDRIRELEEAMAGERDKFRKMLDAKEQEMTEMRDVMQQQLAEYQELLDVKLALDMEINAYRKLLEGEEERLKLSPSPSSRVTVSRATSSSSGSLSATGRLGRSKRKRLEVEEPLGSGPSVLGTGTGGSGGFHLAQQASASGSVSIEEIDLEGKFVQLKNNSDKDQSLGNWRIKRQVLEGEEIAYKFTPKYILRAGQMVTVWAAGAGVAHSPPSTLVWKGQSSWGTGESFRTVLVNADGEEVAMRTVKKSSVMRENENGEEEEEEAEFGEEDLFHQQGDPRTTSRGCYVM 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | lamin B2 |
| 别名 | LMNB2, LAMB2, Lamin-B2, LMN2 |
| 计算分子量 | 68 kDa |
| 观测分子量 | 66-68 kDa |
| GenBank蛋白编号 | BC006551 |
| 基因名称 | Lamin B2 |
| Gene ID (NCBI) | 84823 |
| RRID | AB_2136411 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q03252 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Lamins are nuclear membrane structural components that are important in structural integrity of the nucleus and may also interact with chromatin (PMID: 33033404). Research studies show that lamin B2 knockout mice exhibit neuronal developmental defects and that both proteins are essential for typical brain development (PMID: 20145110). Mutations in Lamin B2 can result in a susceptibility to developing acquired partial lipodystrophy, a rare disorder characterized by the progressive loss of subcutaneous fat in a bilaterally symmetrical fashion (PMID: 16826530).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Lamin B2 antibody 10895-1-AP | Download protocol |
| IHC protocol for Lamin B2 antibody 10895-1-AP | Download protocol |
| IP protocol for Lamin B2 antibody 10895-1-AP | Download protocol |
| WB protocol for Lamin B2 antibody 10895-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol LAP1 supports nuclear adaptability during constrained melanoma cell migration and invasion
| ||
Mol Cell MLKL activates the cGAS-STING pathway by releasing mitochondrial DNA upon necroptosis induction | ||
Nat Chem Biol Fructose-1,6-bisphosphatase 1 dephosphorylates and inhibits TERT for tumor suppression | ||
Sci Adv 53BP1 orchestrates sequence feature of RAG targets to balance DNA repair outcomes during V(D)J recombination. | ||
Pharmacol Res YEATS domain-containing protein GAS41 regulates nuclear shape by working in concert with BRD2 and the Mediator complex in colorectal cancer | ||
Phytomedicine Forsythoside A mitigates osteoarthritis and inhibits chondrocyte senescence by promoting mitophagy and suppressing NLRP3 inflammasome via the Nrf2 pathway |
