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LMBR1L Polyclonal antibody

LMBR1L Polyclonal Antibody for FC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 12154-1-AP

Print datasheet

Synonyms

KIAA1174, LIMR, LMBR1L, Protein LMBR1L

验证数据展示

  • WB analysis of mouse testis using 12154-1-AP

    WB analysis of mouse testis using 12154-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12154-1-AP (LMBR1L antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 12154-1-AP

    IP experiment of HepG2 using 12154-1-AP

    IP result of anti-LMBR1L (IP:12154-1-AP, 4ug; Detection:12154-1-AP 1:500) with HepG2 cells lysate 3200ug.

  • FC experiment of A549 using 12154-1-AP

    FC experiment of A549 using 12154-1-AP

    1X10^6 A549 cells were stained with 0.2ug LMBR1L antibody (12154-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

经过测试的应用

Positive WB detected inmouse testis tissue, A549 cells, HepG2 cells, mouse liver tissue, rat testis tissue
Positive IP detected inHepG2 cells
Positive FC detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12154-1-AP targets LMBR1L in WB, IP, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IP, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen LMBR1L fusion protein Ag2787 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name limb region 1 homolog (mouse)-like
Synonyms KIAA1174, LIMR, LMBR1L, Protein LMBR1L
Calculated Molecular Weight 489 aa, 55 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC015015
Gene Symbol LMBR1L
Gene ID (NCBI) 55716
RRIDAB_2136130
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6UX01
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LMBR1L, also known as LIMR (lipocalin-1-interacting membrane receptor), interacts with lipocalin-1 (Lcn-1), a lipocalin member produced by a number of secretoric glands and tissues and known to bind a variety of lipophilic compounds. LMBR1L is a 489-amino acid protein with a predicted molecular mass of 55 kD and 9 putative transmembrane helices. LMBR1L has been shown to mediate cellular uptake of LMBR1L, thus it is characterized as a endocytic receptor (PMID: 11287427; 12591932). Alternatively spliced transcript variants encoding different isoforms have been described.

实验方案

Product Specific Protocols
WB protocol for LMBR1L antibody 12154-1-APDownload protocol
IP protocol for LMBR1L antibody 12154-1-APDownload protocol
FC protocol for LMBR1L antibody 12154-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols