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验证数据展示

  • WB analysis of mouse testis using 12154-1-AP

    WB analysis of mouse testis using 12154-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12154-1-AP (LMBR1L antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 12154-1-AP

    IP experiment of HepG2 using 12154-1-AP

    IP result of anti-LMBR1L (IP:12154-1-AP, 4ug; Detection:12154-1-AP 1:500) with HepG2 cells lysate 3200ug.

LMBR1L Polyclonal antibody

LMBR1L Polyclonal Antibody for WB, IP, ELISA
Cat No. 12154-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

KIAA1174, LIMR, LMBR1L, Protein LMBR1L

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, A549 cells, HepG2 cells, mouse liver tissue, rat testis tissue
Positive IP detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12154-1-AP targets LMBR1L in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 LMBR1L fusion protein Ag2787 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 limb region 1 homolog (mouse)-like
别名 KIAA1174, LIMR, LMBR1L, Protein LMBR1L
计算分子量 489 aa, 55 kDa
观测分子量 57 kDa
GenBank蛋白编号BC015015
基因名称 LMBR1L
Gene ID (NCBI) 55716
RRIDAB_2136130
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6UX01
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LMBR1L, also known as LIMR (lipocalin-1-interacting membrane receptor), interacts with lipocalin-1 (Lcn-1), a lipocalin member produced by a number of secretoric glands and tissues and known to bind a variety of lipophilic compounds. LMBR1L is a 489-amino acid protein with a predicted molecular mass of 55 kD and 9 putative transmembrane helices. LMBR1L has been shown to mediate cellular uptake of LMBR1L, thus it is characterized as a endocytic receptor (PMID: 11287427; 12591932). Alternatively spliced transcript variants encoding different isoforms have been described.

实验方案

Product Specific Protocols
WB protocol for LMBR1L antibody 12154-1-APDownload protocol
IP protocol for LMBR1L antibody 12154-1-APDownload protocol
FC protocol for LMBR1L antibody 12154-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols