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验证数据展示

  • WB analysis using 15993-1-AP

    WB analysis using 15993-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15993-1-AP (LLGL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 15993-1-AP

    IF Staining of HepG2 using 15993-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LLGL2 antibody (15993-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

LLGL2 Polyclonal antibody

LLGL2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 15993-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

LLGL scribble cell polarity complex component 2, Lethal(2) giant larvae protein homolog 2, HGL

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, K-562 cells, MCF-7 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15993-1-AP targets LLGL2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag8867

Product name: Recombinant human LLGL2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-356 aa of BC010879

Sequence: MRRFLRPGHDPVRERLKRDLFQFNKTVEHGFPHQPSALGYSPSLRILAIGTRSGAIKLYGAPGVEFMGLHQENNAVTQIHLLPGQCQLVTLLDDNSLHLWSLKVKGGASELQEDESFTLRGPPGAAPSATQITVVLPHSSCELLYLGTESGNVFVVQLPAFRALEDRTISSDAVLQRLPEEARHRRVFEMVEALQEHPRDPNQILIGYSRGLVVIWDLQGSRVLYHFLSSQQLENIWWQRDGRLLVSCHSDGSYCQWPVSSEAQQPEPLRSLVPYGPFPCKAITRILWLTTRQGLPFTIFQGGMPRASYGDRHCISVIHDGQQTAFDFTSRVIGFTVLTEADPAASRRASGVGAQG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lethal giant larvae homolog 2 (Drosophila)
别名 LLGL scribble cell polarity complex component 2, Lethal(2) giant larvae protein homolog 2, HGL
计算分子量356aa,40 kDa; 1020aa,113 kDa
观测分子量113-120 kDa
GenBank蛋白编号BC010879
基因名称 LLGL2
Gene ID (NCBI) 3993
RRIDAB_3085478
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6P1M3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lethal giant larvae (Lgl) is a scaffolding protein that regulates the establishment of apical-basal polarity in epithelial cells. The human homologs of Lgl are known as LLGL1 and LLGL2. LLGL1 is a tumor suppressor in multiple human cancers, including hepatocellular carcinoma, malignant melanoma, and colorectal cancer (PMID: 34178676; 15735678; 16170365). LLGL2 functions as a promoter of tumor growth and not as a tumor suppressor in ER+ breast cancer (PMID: 30996345).

实验方案

Product Specific Protocols
IF protocol for LLGL2 antibody 15993-1-APDownload protocol
WB protocol for LLGL2 antibody 15993-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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