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验证数据展示

  • WB analysis using 22496-1-AP

    WB analysis using 22496-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 22496-1-AP (LLGL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 22496-1-AP

    IF Staining of HepG2 using 22496-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LLGL1 antibody (22496-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

LLGL1 Polyclonal antibody

LLGL1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 22496-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

lethal giant larvae homolog 1 (Drosophila), Hugl-1, Hugl1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22496-1-AP targets LLGL1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 LLGL1 fusion protein Ag18057 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lethal giant larvae homolog 1 (Drosophila)
别名 lethal giant larvae homolog 1 (Drosophila), Hugl-1, Hugl1
计算分子量 1064 aa, 115 kDa
观测分子量115 kDa
GenBank蛋白编号BC151838
基因名称 LLGL1
Gene ID (NCBI) 3996
RRIDAB_3669400
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ15334
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lethal giant larvae (Lgl) is a scaffolding protein that regulates the establishment of apical-basal polarity in epithelial cells. The human homologs of Lgl are known as LLGL1 and LLGL2. LLGL1 is a tumor suppressor in multiple human cancers, including hepatocellular carcinoma, malignant melanoma, and colorectal cancer (PMID: 34178676; 15735678; 16170365). LLGL2 functions as a promoter of tumor growth and not as a tumor suppressor in ER+ breast cancer (PMID: 30996345).

实验方案

Product Specific Protocols
WB protocol for LLGL1 antibody 22496-1-APDownload protocol
IF protocol for LLGL1 antibody 22496-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols