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LINGO1 Polyclonal antibody

LINGO1 Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, WB, ELISA

Conjugate

Unconjugated

Cat No : 19097-1-AP

Print datasheet

Synonyms

LERN1, LINGO1, LRRN6A, UNQ201

验证数据展示

  • WB analysis of mouse brain using 19097-1-AP

    WB analysis of mouse brain using 19097-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 19097-1-AP (LINGO1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • FC experiment of SH-SY5Y using 19097-1-AP

    FC experiment of SH-SY5Y using 19097-1-AP

    1X10^6 SH-SY5Y cells were stained with 0.2ug LINGO1 antibody (19097-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive FC detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19097-1-AP targets LINGO1 in WB, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen LINGO1 fusion protein Ag13553 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name leucine rich repeat and Ig domain containing 1
Synonyms LERN1, LINGO1, LRRN6A, UNQ201
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 83 kDa
GenBank Accession NumberBC011057
Gene Symbol LINGO1
Gene ID (NCBI) 84894
RRIDAB_10638484
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96FE5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LINGO-1 is a transmembrane receptor that contains 12 leucine-rich repeats and an immunoglobulin domain. It is expressed primarily in the central nervous system. LINGO-1 binds Nogo-66 receptor (NgR) and p75 and that is a functional component of the NgR/p75 signaling complex (PMID: 14966521). This protein has a calculated molecular weight of 70 kDa and migrates at higher molecular weight due to glycosylation (PMID: 21872576; 25257685; 18326721).

实验方案

Product Specific Protocols
WB protocol for LINGO1 antibody 19097-1-APDownload protocol
FC protocol for LINGO1 antibody 19097-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols