验证数据展示
经过测试的应用
| Positive WB detected in | MDA-MB-231 cells, NIH/3T3 cells, mouse testis tissue, rat testis tissue |
| Positive IP detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17882-1-AP targets LIN9 in WB, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag11889 Product name: Recombinant human LIN9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-296 aa of BC045625 Sequence: MHRGGQPLKKRRGSFKMAELDQLPDESSSAKALVSLKEGSLSNTWNEKYSSLQKTPVWKGRNTSSAVEMPFRNSKRSRLFSDEDDRQINTRSPKRNQRVAMVPQKFTATMSTPDKKASQKIGFRLRNLLKLPKAHKWCIYEWFYSNIDKPLFEGDNDFCVCLKESFPNLKTRKLTRVEWGKIRRLMGKPRRCSSAFFEEERSALKQKRQKIRLLQQRKVADVSQFKDLPDEIPLPLVIGTKVTARLRGVHDGLFTGQIDAVDTLNATYRVTFDRTGLGTHTIPDYEVLSNEPHETM 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | lin-9 homolog (C. elegans) |
| 别名 | TGS, BARA, Beta subunit-associated regulator of apoptosis, hLin-9, HuLin-9 |
| 计算分子量 | 558 aa, 64 kDa |
| 观测分子量 | 55-65 kDa |
| GenBank蛋白编号 | BC045625 |
| 基因名称 | LIN9 |
| Gene ID (NCBI) | 286826 |
| RRID | AB_10859819 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5TKA1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LIN9, also named as BARA or TGS, is a 542 amino acid protein, which belongs to the lin9 family. LIN9 localizes in the nucleus and is expressed in thymus and testis. LIN9 acts as a tumor suppressor and inhibits DNA synthesis. LIN9 plays a role in the expression of genes, which required for the G1/S transition.
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for LIN9 antibody 17882-1-AP | Download protocol |
| WB protocol for LIN9 antibody 17882-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Dis Targeting FBXO22 enhances radiosensitivity in non-small cell lung cancer by inhibiting the FOXM1/Rad51 axis | ||
Int J Oncol Transcription factor E2F4 facilitates SUMOylation to promote HCC progression through interaction with LIN9 | ||
EMBO Mol Med Interactions between BRD4S, LOXL2, and MED1 drive cell cycle transcription in triple-negative breast cancer | ||
FEBS Open Bio The dual role of the DREAM/G2M pathway in non-tumorigenic immortalization of senescent cells | ||
J Transl Med Vascular restenosis following paclitaxel-coated balloon therapy is attributable to NLRP3 activation and LIN9 upregulation
|
