LIN9 Polyclonal antibody
LIN9 Polyclonal Antibody for IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | MDA-MB-231 cells, NIH/3T3 cells, mouse testis tissue, rat testis tissue |
| Positive IP detected in | HEK-293 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
17882-1-AP targets LIN9 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IP, WB, ELISA |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | LIN9 fusion protein Ag11889 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | lin-9 homolog (C. elegans) |
| Synonyms | BARA, BARPsv, hLin 9, HuLin 9, Lin 9, lin 9 homolog (C. elegans), LIN9, pRB associated protein, Protein lin 9 homolog, TGS, TGS1, TUDOR gene similar protein |
| Calculated Molecular Weight | 558 aa, 64 kDa |
| Observed Molecular Weight | 55-65 kDa |
| GenBank Accession Number | BC045625 |
| Gene Symbol | LIN9 |
| Gene ID (NCBI) | 286826 |
| RRID | AB_10859819 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5TKA1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LIN9, also named as BARA or TGS, is a 542 amino acid protein, which belongs to the lin9 family. LIN9 localizes in the nucleus and is expressed in thymus and testis. LIN9 acts as a tumor suppressor and inhibits DNA synthesis. LIN9 plays a role in the expression of genes, which required for the G1/S transition.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LIN9 antibody 17882-1-AP | Download protocol |
| IP protocol for LIN9 antibody 17882-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO Mol Med Interactions between BRD4S, LOXL2, and MED1 drive cell cycle transcription in triple-negative breast cancer | ||
FEBS Open Bio The dual role of the DREAM/G2M pathway in non-tumorigenic immortalization of senescent cells |