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验证数据展示

  • WB analysis of rat brain using 25150-1-AP

    WB analysis of rat brain using 25150-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 25150-1-AP (LIN7A Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

  • IP experiment of mouse brain using 25150-1-AP

    IP experiment of mouse brain using 25150-1-AP

    IP result of anti-LIN7A (IP:25150-1-AP, 3ug; Detection:25150-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

  • IHC staining of mouse brain using 25150-1-AP

    IHC staining of mouse brain using 25150-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25150-1-AP (LIN7A Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of mouse brain using 25150-1-AP

    IHC staining of mouse brain using 25150-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25150-1-AP (LIN7A Antibody) at dilution of 1:50 (under 40x lens).

LIN7A Polyclonal antibody

LIN7A Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 25150-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA and More (1)

别名

hLin 7, lin 7 homolog A (C. elegans), LIN 7A, LIN7, LIN7A, MALS 1, MALS1, Mammalian lin seven protein 1, Protein lin 7 homolog A, Tax interaction protein 33, TIP 33, Veli 1, VELI1, Vertebrate lin 7 homolog 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

25150-1-AP targets LIN7A in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用IF
经测试反应性 human, mouse, rat
文献引用反应性rat
免疫原 LIN7A fusion protein Ag18440 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lin-7 homolog A (C. elegans)
别名 hLin 7, lin 7 homolog A (C. elegans), LIN 7A, LIN7, LIN7A, MALS 1, MALS1, Mammalian lin seven protein 1, Protein lin 7 homolog A, Tax interaction protein 33, TIP 33, Veli 1, VELI1, Vertebrate lin 7 homolog 1
计算分子量 233 aa, 26 kDa
观测分子量 28-30 kDa
GenBank蛋白编号BC118609
基因名称 LIN7A
Gene ID (NCBI) 8825
RRIDAB_2879925
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO14910
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LIN7A antibody 25150-1-APDownload protocol
IHC protocol for LIN7A antibody 25150-1-APDownload protocol
IP protocol for LIN7A antibody 25150-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratIF

iScience

Induced retinal pigment epithelial cells with anti-epithelial-to-mesenchymal transition ability delay retinal degeneration

Authors - Haibin Tian
View Article