验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, DU 145 cells, NIH/3T3 cells |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
55003-1-AP targets LIN41 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tripartite motif-containing 71 |
| 别名 | TRIM71, RING-type E3 ubiquitin transferase TRIM71, Protein lin-41 homolog, Protein lin 41 homolog, LIN 41 |
| 计算分子量 | 93 kDa |
| 观测分子量 | 93 kDa |
| GenBank蛋白编号 | NM_001039111 |
| 基因名称 | LIN41 |
| Gene ID (NCBI) | 131405 |
| RRID | AB_2616313 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q2Q1W2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TRIM71, also named as LIN41, belongs to the TRIM/RBCC family. It may be involved in controlling the timing of organ formation during development. The antibody is specific to TRIM71.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for LIN41 antibody 55003-1-AP | Download protocol |
| WB protocol for LIN41 antibody 55003-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Cycle TRIM71 binds to IMP1 and is capable of positive and negative regulation of target RNAs. | ||
Cell Mol Gastroenterol Hepatol HDLBP promotes hepatocellular carcinoma proliferation and sorafenib resistance by suppressing Trim71-dependent RAF1 degradation | ||
Mol Carcinog Circ_0007429/miR-637/TRIM71/Ago2 axis participates in the regulation of proliferation, migration, invasion, apoptosis, and aerobic glycolysis of HCC |
