验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, Jurkat cells |
| Positive IF/ICC detected in | A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
26651-1-AP targets LIN37 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag24565 Product name: Recombinant human LIN37 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-93 aa of BC009071 Sequence: MFPVKVKVEKSELEMAKARNQLDAVLQCLLEKSHMDRERLDEEAGKTPSDTHNKDCSIAATGKRPSARFPHQRRKKRREMDDGLAEGGPQRSN 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | lin-37 homolog (C. elegans) |
| 别名 | Protein lin-37 homolog, Protein lin 37 homolog, MSTP064, lin 37, F25965 |
| 观测分子量 | 36 kDa |
| GenBank蛋白编号 | BC009071 |
| 基因名称 | LIN37 |
| Gene ID (NCBI) | 55957 |
| RRID | AB_2880590 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96GY3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LIN37, also named as Antolefinin and MSTP064, is a component of the DREAM complex (also named LINC complex). LIN37 migrates to 37 kDa for modification.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for LIN37 antibody 26651-1-AP | Download protocol |
| WB protocol for LIN37 antibody 26651-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
