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LIN37 Polyclonal antibody

LIN37 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 26651-1-AP

Print datasheet

Synonyms

Antolefinin, F25965, lin 37, lin 37 homolog (C. elegans), LIN37, Protein lin 37 homolog, ZK418.4

验证数据展示

  • WB analysis using 26651-1-AP

    WB analysis using 26651-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26651-1-AP (LIN37 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 26651-1-AP

    IF Staining of A549 using 26651-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using 26651-1-AP (LIN37 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells, Jurkat cells
Positive IF detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26651-1-AP targets LIN37 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity human
Immunogen LIN37 fusion protein Ag24565 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lin-37 homolog (C. elegans)
Synonyms Antolefinin, F25965, lin 37, lin 37 homolog (C. elegans), LIN37, Protein lin 37 homolog, ZK418.4
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC009071
Gene Symbol LIN37
Gene ID (NCBI) 55957
RRIDAB_2880590
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96GY3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LIN37, also named as Antolefinin and MSTP064, is a component of the DREAM complex (also named LINC complex). LIN37 migrates to 37 kDa for modification.

实验方案

Product Specific Protocols
WB protocol for LIN37 antibody 26651-1-APDownload protocol
IF protocol for LIN37 antibody 26651-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols