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验证数据展示

  • WB analysis using 26651-1-AP

    WB analysis using 26651-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26651-1-AP (LIN37 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 26651-1-AP

    IF Staining of A549 using 26651-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using LIN37 antibody (26651-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green).

LIN37 Polyclonal antibody

LIN37 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 26651-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Protein lin-37 homolog, Protein lin 37 homolog, MSTP064, lin 37, F25965

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, Jurkat cells
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

26651-1-AP targets LIN37 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
免疫原 LIN37 fusion protein Ag24565 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lin-37 homolog (C. elegans)
别名 Protein lin-37 homolog, Protein lin 37 homolog, MSTP064, lin 37, F25965
观测分子量 36 kDa
GenBank蛋白编号BC009071
基因名称 LIN37
Gene ID (NCBI) 55957
RRIDAB_2880590
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96GY3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LIN37, also named as Antolefinin and MSTP064, is a component of the DREAM complex (also named LINC complex). LIN37 migrates to 37 kDa for modification.

实验方案

Product Specific Protocols
WB protocol for LIN37 antibody 26651-1-APDownload protocol
IF protocol for LIN37 antibody 26651-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Int J Oncol

Transcription factor E2F4 facilitates SUMOylation to promote HCC progression through interaction with LIN9

Authors - Zhenwei Ma
View Article