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CoraLite® Plus 488-conjugated LIN28 Monoclonal antibody

LIN28 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4F5G6

Cat No : CL488-60344

Print datasheet

Synonyms

CSDD1, FLJ12457, LIN 28, lin 28 homolog (C. elegans), Lin 28A, LIN28, Lin28A, Protein lin 28 homolog A, ZCCHC1

验证数据展示

  • IF Staining of human prostate cancer using CL488-60344

    IF Staining of human prostate cancer using CL488-60344

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CoraLite® Plus 488 LIN28 antibody (CL488-60344, Clone: 4F5G6 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inhuman prostate cancer tissue
For other applications, we recommend the unconjugated version of this antibody, 60344-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60344 targets LIN28 in IF applications and shows reactivity with human samples.

Tested Applications IF
Tested Reactivity human
Immunogen LIN28 fusion protein Ag2312 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name lin-28 homolog (C. elegans)
Synonyms CSDD1, FLJ12457, LIN 28, lin 28 homolog (C. elegans), Lin 28A, LIN28, Lin28A, Protein lin 28 homolog A, ZCCHC1
Calculated Molecular Weight 209 aa, 23 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC028566
Gene Symbol LIN28
Gene ID (NCBI) 79727
RRIDAB_2919251
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9H9Z2
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

LIN28 is one of the four key human factors (OCT4, SOX2, NANOG and LIN28) used to reprogram human fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (Ips) cell. Lin28 is a marker of undifferentiated human embryonic stem cells and a cytoplasmic Mrna-binding protein that binds to and enhances the translation of the IGF2 Mrna. LIN28 has also been shown to bind to the let-7 pre-miRNA and block production of the mature let-7 microRNA in mouse embryonic stem cells. The calculated molecular weight of LIN28 is 23 kDa, but the modified LIN28 is about 28 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 LIN28 antibody CL488-60344Download protocol
Standard Protocols
Click here to view our Standard Protocols