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LIN28 Monoclonal antibody, PBS Only

LIN28 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

4F5G6

Cat No : 60344-1-PBS

Print datasheet

Synonyms

CSDD1, LIN 28, lin 28 homolog (C. elegans), Lin 28A, LIN28, Lin28A, Protein lin 28 homolog A, ZCCHC1

验证数据展示

  • IF Staining of human prostate cancer using 60344-1-Ig (same clone as 60344-1-PBS)

    IF Staining of human prostate cancer using 60344-1-Ig (same clone as 60344-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using LIN28 antibody (60344-1-Ig, Clone: 4F5G6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60344-1-PBS in a different storage buffer formulation.

  • WB analysis of NCCIT using 60344-1-Ig (same clone as 60344-1-PBS)

    WB analysis of NCCIT using 60344-1-Ig (same clone as 60344-1-PBS)

    NCCIT cell were subjected to SDS PAGE followed by western blot with 60344-1-Ig (LIN28 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60344-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 60344-1-Ig (same clone as 60344-1-PBS)

    IHC staining of human prostate cancer using 60344-1-Ig (same clone as 60344-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60344-1-Ig (LIN28 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60344-1-PBS in a different storage buffer formulation.

产品信息

60344-1-PBS targets LIN28 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB,Indirect ELISA,IHC,IF
Tested Reactivity human
Immunogen LIN28 fusion protein Ag2312 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name lin-28 homolog (C. elegans)
Synonyms CSDD1, LIN 28, lin 28 homolog (C. elegans), Lin 28A, LIN28, Lin28A, Protein lin 28 homolog A, ZCCHC1
Calculated Molecular Weight 209 aa, 23 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC028566
Gene Symbol LIN28
Gene ID (NCBI) 79727
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9H9Z2
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

LIN28 is one of the four key human factors (OCT4, SOX2, NANOG and LIN28) used to reprogram human fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (Ips) cell. Lin28 is a marker of undifferentiated human embryonic stem cells and a cytoplasmic Mrna-binding protein that binds to and enhances the translation of the IGF2 Mrna. LIN28 has also been shown to bind to the let-7 pre-miRNA and block production of the mature let-7 microRNA in mouse embryonic stem cells. The calculated molecular weight of LIN28 is 23 kDa, but the modified LIN28 is about 28 kDa.