- Featured Product
- KD/KO Validated
LIMS1 Polyclonal antibody
LIMS1 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC, IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | Caco-2 cells, mouse lung tissue, human kidney tissue, HeLa cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human oesophagus cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | Hela cells |
| Positive FC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of 20772-1-AP is LIMS1 Fusion Protein expressed in E. coli.
| Tested Applications | FC, IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | WB, IP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | LIMS1 fusion protein Ag14764 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | LIM and senescent cell antigen-like domains 1 |
| Synonyms | LIMS1, PINCH, PINCH 1, PINCH1 |
| Calculated Molecular Weight | 325 aa, 37 kDa |
| Observed Molecular Weight | 35-37 kDa |
| GenBank Accession Number | BC005341 |
| Gene Symbol | LIMS1 |
| Gene ID (NCBI) | 3987 |
| RRID | AB_10695765 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P48059 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LIMS1 antibody 20772-1-AP | Download protocol |
| IHC protocol for LIMS1 antibody 20772-1-AP | Download protocol |
| IF protocol for LIMS1 antibody 20772-1-AP | Download protocol |
| IP protocol for LIMS1 antibody 20772-1-AP | Download protocol |
| FC protocol for LIMS1 antibody 20772-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun PINCH-1 regulates mitochondrial dynamics to promote proline synthesis and tumor growth.
| ||
J Cell Biol A PINCH-1-Smurf1 signaling axis mediates mechano-regulation of BMPR2 and stem cell differentiation.
| ||
Theranostics PINCH-1 promotes IGF-1 receptor expression and skin cancer progression through inhibition of the GRB10-NEDD4 complex. | ||
J Cell Sci Differences in binding to the ILK complex determines kindlin isoform adhesion localization and integrin activation. | ||
Amino Acids PINCH-1 promotes Δ1-pyrroline-5-carboxylate synthase expression and contributes to proline metabolic reprogramming in lung adenocarcinoma.
| ||
Cancer Med The RNA-binding protein RBMS3 inhibits the progression of colon cancer by regulating the stability of LIMS1 mRNA |
