验证数据展示
经过测试的应用
| Positive WB detected in | U-937 cells, Raji cells |
| Positive IHC detected in | human ovary cancer tissue, human brain tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15471-1-AP targets LIMD2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag7775 Product name: Recombinant human LIMD2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-127 aa of BC004400 Sequence: MFQAAGAAQATPSHDAKGGGSSTVQRSKSFSLRAQVKETCAACQKTVYPMERLVADKLIFHNSCFCCKHCHTKLSLGSYAALHGEFYCKPHFQQLFKSKGNYDEGFGRKQHKELWAHKEVDPGTKTA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | LIM domain containing 2 |
| 别名 | LIM domain containing 2, LIM domain-containing protein 2, SB143 |
| 计算分子量 | 14 kDa |
| 观测分子量 | 16-20 kDa |
| GenBank蛋白编号 | BC004400 |
| 基因名称 | LIMD2 |
| Gene ID (NCBI) | 80774 |
| RRID | AB_10859817 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BT23 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for LIMD2 antibody 15471-1-AP | Download protocol |
| WB protocol for LIMD2 antibody 15471-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Bioengineered LIM domain-containing 2 (LIMD2) promotes the progress of ovarian cancer via the focal adhesion signaling pathway. | ||
Mol Biol Rep Knockdown of LIMD2 inhibits the progression of ovarian carcinoma through ERK1/2 pathway
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