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验证数据展示

  • WB analysis of U-937 using 15471-1-AP

    WB analysis of U-937 using 15471-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 15471-1-AP (LIMD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 15471-1-AP

    IHC staining of human liver cancer using 15471-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 15471-1-AP (LIMD2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver cancer using 15471-1-AP

    IHC staining of human liver cancer using 15471-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 15471-1-AP (LIMD2 antibody) at dilution of 1:50 (under 40x lens).

  • Featured Product
  • KD/KO验证

LIMD2 Polyclonal antibody

LIMD2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15471-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA and More (1)

别名

LIM domain containing 2, LIMD2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU-937 cells, Raji cells
Positive IHC detected inhuman liver cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15471-1-AP targets LIMD2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 LIMD2 fusion protein Ag7775 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 LIM domain containing 2
别名 LIM domain containing 2, LIMD2
计算分子量 14 kDa
观测分子量 16-20 kDa
GenBank蛋白编号BC004400
基因名称 LIMD2
Gene ID (NCBI) 80774
RRIDAB_10859817
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BT23
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LIMD2 antibody 15471-1-APDownload protocol
IHC protocol for LIMD2 antibody 15471-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Bioengineered

LIM domain-containing 2 (LIMD2) promotes the progress of ovarian cancer via the focal adhesion signaling pathway.

Authors - Lixin Chen
View Article
humanWB

Mol Biol Rep

Knockdown of LIMD2 inhibits the progression of ovarian carcinoma through ERK1/2 pathway

Authors - Haiyang Hu
  • KD Validated
View Article