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LILRB1 Polyclonal antibody

LILRB1 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 26455-1-AP

Print datasheet

Synonyms

CD85, CD85J, ILT 2, ILT2, LILRB1, LIR 1, LIR1, MIR 7, MIR7

验证数据展示

  • WB analysis using 26455-1-AP

    WB analysis using 26455-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26455-1-AP (LILRB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 26455-1-AP

    IHC staining of human liver using 26455-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26455-1-AP (LILRB1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of Ramos using 26455-1-AP

    FC experiment of Ramos using 26455-1-AP

    1X10^6 Ramos cells were stained with 0.20 ug Anti-Human LILRB1 (26455-1-AP) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000. Fixed with90% MeOH.

经过测试的应用

Positive WB detected inDaudi cells, Ramos cells
Positive IHC detected inhuman liver tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inRamos cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC)FC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

26455-1-AP targets LILRB1 in WB, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, IHC, WB, ELISA
Cited ApplicationsIHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen LILRB1 fusion protein Ag24795 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1
Synonyms CD85, CD85J, ILT 2, ILT2, LILRB1, LIR 1, LIR1, MIR 7, MIR7
Calculated Molecular Weight 71 kDa
Observed Molecular Weight 71 and 110 kDa
GenBank Accession NumberBC015731
Gene Symbol LILRB1
Gene ID (NCBI) 10859
RRIDAB_2880518
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NHL6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LILRB1, also named as CD85J or ILT2, includes four C2 Ig domains binding HLA class I molecules in the extracellular region and a cytoplasmic domain containing four immunoreceptor tyrosine-based inhibition motifs (ITIM). LILRB1 is a surface glycoprotein detected on the surface of B cells, monocytes, dendritic cells, T cells and NK cells. The predicted MW of LILRB1 is 71 kDa and 26455-1-AP antibody recognizes the 71 and 110 kDa which might be the noncovalently linked monomer. (PMID:17601702; 15474475; 17549736; 24098421)

实验方案

Product Specific Protocols
WB protocol for LILRB1 antibody 26455-1-APDownload protocol
IHC protocol for LILRB1 antibody 26455-1-APDownload protocol
FC protocol for LILRB1 antibody 26455-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Int Immunopharmacol

LILRB1+ immune cell infiltration identifies immunosuppressive microenvironment and dismal outcomes of patients with ovarian cancer

Authors - Xiaoyu Xu
View Article