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验证数据展示

  • WB analysis of K-562 using 18821-1-AP

    WB analysis of K-562 using 18821-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 18821-1-AP (LILRA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

LILRA5/CD85f Polyclonal antibody

LILRA5/CD85f Polyclonal Antibody for WB, ELISA
Cat No. 18821-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

LILRA5, CD85, CD85 antigen-like family member F, CD85f, ILT 11

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18821-1-AP targets LILRA5/CD85f in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 5
别名 LILRA5, CD85, CD85 antigen-like family member F, CD85f, ILT 11
计算分子量 299 aa, 33 kDa
观测分子量 31-33 kDa
GenBank蛋白编号BC153203
基因名称 LILRA5
Gene ID (NCBI) 353514
RRIDAB_2281279
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDA6NI73
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LILRA5, also named as CD85f, ILT11, LILRB7 or LIR9, is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is a group of immune inhibitory and stimulatory receptors (PMID: 15304001). There are two membrane-bound and two soluble forms of LILRA5. The transmembrane LILRA5 contains a short cytoplasmic domain and a charged arginine residue within the transmembrane region (PMID: 19009525). It is mostly expressed in myeloid cells, including monocytes and neutrophils (PMID: 12393390). LILRA5 may play a role in triggering innate immune responses. It does not function in class I MHC antigens recognition (PMID: 16675463). The antibody recognizes the isoform 1 and isoform 2 of LILRA5.

实验方案

Product Specific Protocols
WB protocol for LILRA5/CD85f antibody 18821-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols